Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Amyloid-&beta1; 42 Detection Kit Standard
HTRF Human Amyloid-&beta1; 42 Detection Kit Standard
Molecular Devices Filter Lens, 405 nm, Optical, Microplate Reader Compatible
Molecular Devices 405 nm filter lens is an optical bandpass filter designed for filter-based microplate readers. It transmits light centered at 405 nm for selective detection in absorbance or fluorescence assays and serves as a replaceable filter accessory compatible with common filter-based readers such as EMax, VMax, UVMax, and ThermoMax models.
- Center wavelength 405 nm.
- Optical bandpass filter lens for filter-based detection.
- Replaceable accessory for microplate readers.
- Suitable for assays requiring detection near 405 nm.
Revvity Health Sciences Inc HTRF Human Total CDK1 Detection Kit, 500 Assay Points
HTRF Human Total CDK1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-RAB10 (Thr73) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-RAB10 (Thr73) Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Total Myd88 Detection Kit, 10,000 Assay Points
HTRF Total Myd88 Detection Kit, 10,000 Assay Points
Molecular Devices Fluorescence Excitation Filter, 370 nm, Time-Resolved, Single Unit
Molecular Devices 370 nm time-resolved fluorescence filter provides excitation at 370 nm for time-resolved fluorescence assays. It is supplied as a single replacement optical filter for compatible fluorescence instruments.
- Provides excitation centered at 370 nm.
- Designed for time-resolved fluorescence measurements.
- Supplied as a single replacement optical filter.
- Intended for use in compatible fluorescence detection instruments.
- Helps improve excitation specificity for time-resolved assays.
Molecular Devices Absorbance Filter, 640 nm, 8 nm BW
The AD 340 absorbance photometric filter provides a narrowband 640 nm passband for absorbance measurements in filter-wheel microplate readers. It is supplied as a single replacement filter for compatible readers and supports assays that require red-wavelength detection.
- Absorbance detection at 640 nm center wavelength.
- Bandwidth 8 nm.
- Designed for filter-wheel microplate reader configurations.
- Single replacement filter for maintenance and assay reproducibility.
- Supports red-wavelength absorbance assays and endpoint measurements.
Molecular Devices Fluorescence Polarization Filter, 535 nm, 25 nm, Vertical
Fluorescence polarization filter optimized for fluorescence polarization assays. The filter has a 535 nm center wavelength with a 25 nm bandwidth and a vertical polarization orientation. It includes a holder for mounting in compatible microplate readers and is intended for use with FP measurement configurations.
- Optimized for fluorescence polarization measurements.
- Center wavelength 535 nm, bandwidth 25 nm.
- Vertical polarization orientation for polarization-based assays.
- Supplied with a holder for secure mounting in reader filter positions.
- Compatible with SpectraMax and similar microplate readers that accept filter holders.
Revvity Health Sciences Inc HTRF Human Total FGFR4 Detection Kit, Control Lysate
HTRF Human Total FGFR4 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points
HTRF Human Total EGFR DEL19 Detection Kit, 50,000 Assay Points