Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Molecular Devices SpectraCuvette Adapter, 18 mm, SpectraMax compatible
The Molecular Devices SpectraCuvette Adapter (part number 5301193) adapts standard cuvettes for measurement in compatible microplate readers by positioning cuvettes at a defined plate height for accurate optical alignment and absorbance readings.
- Provides 18 mm plate height for proper optical alignment.
- Holds cuvettes securely for absorbance measurements.
- Designed to maintain plate height below instrument maximums to prevent damage.
- Uses plate-height marking to ensure correct adapter selection.
Molecular Devices Validation Plate, Absorbance, NIST-Traceable, ISO 17025 Recertifiable
The SpectraTest ABS2 Validation Plate from Molecular Devices is an automated absorbance validation tool used to verify the optical performance and sensitivity of absorbance-capable microplate readers. It provides NIST-traceable photometric checks and supports recertification to maintain GMP and GLP compliance; requires SoftMax Pro software version 6.5.1 or higher.
- Validates absorbance sensitivity and photometric accuracy.
- Automated certificate value entry for streamlined validation workflows.
- Compatible with absorbance-capable microplate readers and automated handling systems.
- Supports annual recertification with ISO 17025 traceability.
- Designed for GMP and GLP instrument performance verification.
- Factory recertification and repair services reduce downtime.
Electron Microscopy Sciences EMS SmartDoc™ 590nm Band Pass Filter for EtBr
For use with EMS SmartDoc gel imaging system.
Dojindo Molecular Technologies Inc ATP Assay Kit-Luminescence (100 tests) is used to determine intracellular levels of ATP by luciferase luminescence assay. High-sensitivity detection can be performed with stable luminescence. 200 tests
Adenosine triphosphate (ATP) is an energy source for living cells and is synthesized by both glycolysis and mitochondrial oxidative phosphorylation. Decreased ATP levels are associated with cancer, aging, neurodegenerative diseases, and mitochondrial diseases. The ATP Assay Kit-Luminescence enables the quantitation of intracellular ATP by luciferase luminescence assay. This kit can be applied for microplate assay. Without a complicated process including medium removal and cell washing, the ATP Assay Kit-Luminescence can generate a stable luminescent signal with a half-life of greater than 3 hours by simply mixing the kit components and adding the reagent. This kit contains an ATP Standard, which provides highly reproducible data without measuring unstable ATP. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Sigma Aldrich Fine Chemicals Biosciences Hellma(R) fluorescence cuvettes, semi Micro Suprasil(R) quartz, spectral range 200-2500 nm, pathlength 10x4 mm, chamber volume 1000 muL | 1EA
Hellma(R) fluorescence cuvettes, semi Micro Suprasil(R) quartz, spectral range 200-2500 nm, pathlength 10x4 mm, chamber volume 1000 muL | 1EA
Revvity Health Sciences Inc HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
HTRF Human Total Estrogen Receptor α Detection Kit, Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total BRD9 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, Control Lysate
HTRF Human Phospho-α-Synuclein (Ser129) Aggregation Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 500 Assay Points
HTRF Human Phospho-CHK-2 (Thr68) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total RET Detection Kit, 500 Assay Points
HTRF Human & Mouse Total RET Detection Kit, 500 Assay Points
Biotium Aurora B (Proliferation Marker) (AURKB/1521), CF568 conjugate, 0.1mg/mL
This antibody recognizes a protein of 39 kDa, which is identified as Aurora kinase B (or Aurora B). Aurora B is a serine/threonine protein kinase and component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. It is important for accurate chromosome segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachment, and regulation of the mitotic checkpoint. Aurora B forms a tight complex with inner centrosome protein and survivin. Inactivation of any of these proteins causes similar defects in chromosome segregation. A significant overexpression of Aurora B has been found in a variety of human tumors including non-small cell lung carcinoma, astrocytoma, seminoma and carcinomas of the colon, prostate, endometrium and thyroid. The expression level of Aurora B is associated with cell proliferation and prognosis in these tumors.Primary antibodies are available purified, or with
Revvity Health Sciences Inc HTRF Strep-Tactin Tb-Conjugate, 20,000 Assay Points
HTRF Strep-Tactin Tb-Conjugate, 20,000 Assay Points