Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Perkin Elmer US LLC Manual Injector Bracket
Magnetic Clip-on Bracket for Manual Injection on FX series LC Instruments.
Molecular Devices Fluorescence Excitation Filter, 535 nm, 25 nm bandwidth
Molecular Devices 535 nm fluorescence excitation filter (MPN 6590-0057) is a bandpass excitation filter centered at 535 nm with a 25 nm bandwidth, intended for installation in compatible microplate reader filter slides to provide selective excitation for fluorescence assays.
- Excitation bandpass centered at 535 nm with a 25 nm bandwidth.
- Optimized for fluorescence assay excitation to improve signal specificity.
- Form factor designed for installation in compatible microplate reader filter slides.
- Intended for use with multi-mode microplate readers that accept filter slides.
Revvity Health Sciences Inc HTRF Human VHL Binding Kit, 500 Assay Points
HTRF Human VHL Binding Kit, 500 Assay Points
Molecular Devices Photometric Absorbance Filter, 470 nm, 8 nm Bandwidth
The AD 340 photometric filter centered at 470 nm is an absorbance accessory designed for narrowband photometric measurements in microplate assays. It delivers wavelength-specific absorbance detection for filter-based workflows and is intended for compatible multimode microplate readers.
- Absorbance filter for photometric measurements.
- Center wavelength 470 nm.
- Bandwidth approximately 8 nm.
- Designed for use with F3 and F5 multimode microplate readers.
- Suitable for filter-based absorbance assays in microplates.
Revvity Health Sciences Inc HTRF cIAP1 BIR3 Binding Kit, 500 Assay Points
HTRF cIAP1 BIR3 Binding Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK5 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total CDK5 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total RBM39 Detection Kit, Control Lysate
HTRF Human & Mouse Total RBM39 Detection Kit, Control Lysate
Revvity Health Sciences Inc HTRF Human & Mouse Total BRD7 Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Total BRD7 Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc HTRF Human IL-1β High Performance Detection Kit, 96 Assay Points
HTRF Human IL-1β High Performance Detection Kit, 96 Assay Points
Molecular Devices Fluorescence Filter, 400 nm, 30 nm BW, Holder
This fluorescence filter with holder provides an optical band centered at 400 nm with a 30 nm bandwidth for use in microplate-based fluorescence assays, luminescence, and time-resolved fluorescence detection. It serves as an accessory optical element for multimode microplate readers.
- 400 nm center wavelength, 30 nm bandwidth
- Designed for fluorescence, luminescence, and time-resolved fluorescence detection
- Includes mounting holder for installation in standard filter slots
- Intended for use with multimode microplate readers and plate-based assays
Revvity Health Sciences Inc HTRF Human ApoB Detection Kit Standard
HTRF Human ApoB Detection Kit Standard
Research Products International Corp Well Orienter Cards, 4 per Package
The Well Orienter System increases accuracy and efficiency with 96 well micro-plate procedures. The plastic well index card fits perfectly under a micro-plate and clearly identifies each well with distinct alpha/numeric characters.