Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Strep-Tactin d2-Conjugate, 5,000 Assay Points
HTRF Strep-Tactin d2-Conjugate, 5,000 Assay Points
Revvity Health Sciences Inc HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL
HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL
Molecular Devices Time-Resolved Emission Filter, 616 nm, 10 nm Bandwidth
This Molecular Devices time-resolved emission filter provides a 616 nm center wavelength with a 10 nm bandwidth for time-resolved fluorescence (TRF) and TR-FRET detection in multi-mode microplate readers. It is intended to isolate emission bands and improve signal selectivity in time-resolved assays.
- Optimized for time-resolved fluorescence and TR-FRET assays.
- 616 nm center wavelength, 10 nm bandwidth.
- Emission filter configured for use in microplate reader filter holders.
- Improves emission selectivity and reduces background signal.
- Referenced in instrument application notes for optimized TR-FRET settings.
Molecular Devices Fluorescence Polarization Filter, Polarized Vertical, 300-750 nm
Molecular Devices fluorescence polarization filter for fluorescence polarization assays across a broad UV-visible range. The filter is supplied in a vertical polarization configuration and is intended for use in filter-based microplate readers that support fluorescence polarization.
- Broad wavelength range: 300-750 nm.
- Vertical polarization orientation.
- Designed for fluorescence polarization measurements.
- Installs in a filter holder or slide for filter-based microplate readers.
- Suitable for quantitative polarization assays.
Revvity Health Sciences Inc HTRF Human Total CDK7 Detection Kit, 10,000 Assay Points
HTRF Human Total CDK7 Detection Kit, 10,000 Assay Points
Molecular Devices Excitation Filter, 360 nm, 35 nm Bandwidth
The Molecular Devices 360 nm fluorescence excitation filter is an accessory for FilterMax series multi-mode microplate readers, providing a narrow excitation band centered at 360 nm for assays that require near-UV excitation.
- Provides 360 nm excitation with approximately 35 nm bandwidth.
- Designed for fluorescence assays on microplate readers and ELISA readers.
- Fits FilterMax series reader filter holders for standard microplate formats.
- Improves signal-to-noise for near-UV-excited fluorophores.
- Simple to install and replace during routine maintenance.
Electron Microscopy Sciences Pipette Tip Universal, 5 - 200 mcL
Pipette tips are low binding to ensure accuracy and precision. The low binding surface is the result of a manufacturing process that binds a perfluorooctyl polymer to polypropylene resin–resulting in an exposed trifluoromethyl surface. This surface reduces the surface energy of liquid and repels any substance so sample retention and binding are minimized.
Not detectable/Non-reactive.
Extracted only by a fluorinated solvent.
Autoclaving will not compromise performance.
Available in convenient hinged racked package.
Tips and racks are fully autoclavable. Natural tips feature reference marks at 10 ul, 50 ul, and 100 u. Low binding surface.
Range: 5 - 200 µl
Revvity Health Sciences Inc HTRF Mouse Phospho-STING (Ser365) Detection Kit, 500 Assay Points
HTRF Mouse Phospho-STING (Ser365) Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Progranulin Detection Kit, 500 Assay Points
HTRF Human Progranulin Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total HPK1 Detection Kit, 50,000 Assay Points
HTRF Human Total HPK1 Detection Kit, 50,000 Assay Points
Molecular Devices Emission Filter, 710 nm, 50 nm BW, TRF
This optical emission filter is specified at a 710 nm center wavelength with a 50 nm bandwidth and is intended for fluorescence, luminescence, and time-resolved fluorescence (TRF) detection in compatible multi-mode microplate readers.
- Center wavelength 710 nm.
- Bandwidth 50 nm for narrow spectral selection.
- Suitable for fluorescence, luminescence, and TRF assays.
- Accessory filter for compatible multi-mode microplate readers.
- Improves signal-to-noise for near-infrared detection.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points
AlphaLISA SureFire Ultra Human Total TIE2 Detection Kit, 100 Assay Points