Fast 1-Step RT-qPCR Mix, 2x has been designed with the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with a fast hot-start polymerase, reverse transcriptase, RNase Inhibitor, dNTPs and MgCl2, for highly reproducible, accurate, sensitive RT-qPCR efficiency in a single tube, even from low abundance targets and scarce samples. With high processivity and robustness, it delivers excellent results in both singleplex and multiplex RT-qPCR assays and confers superior assay performance under fast thermal cycling conditions, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.