One-Step Reverse Transcription PCR Reagents
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Thermo Scientific™ Verso 1-Step RT-PCR Kit ReddyMix, with ThermoPrime Taq
Thermo Scientific Verso 1-Step RT-PCR Kit ReddyMix with ThermoPrime Taq is a complete set of RT-PCR reagents that combines everything needed for RT-PCR.
New England Biolabs, Inc. OneTaq One-Step RT-PCR Kit – 30 reactions
The OneTaq One-Step RT-PCR Kit offers sensitive and robust end-point detection of RNA templates. cDNA synthesis and PCR amplification steps are performed in a single reaction using gene-specific primers, resulting in a streamlined RT-PCR protocol. The kit combines three optimized mixes: OneTaq One-Step Enzyme Mix, OneTaq One-Step Reaction Mix and OneTaq One-Step Quick-Load Reaction Mix. The Enzyme Mix combines ProtoScript II Reverse Transcriptase, Murine RNase Inhibitor and OneTaq Hot Start DNA Polymerase. ProtoScript II Reverse Transcriptase is a mutant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. OneTaq Hot Start DNA Polymerase is mixture of a Hot Start Taq DNA Polymerase combined with a proof-reading DNA polymerase, resulting in high-yield amplification with minimal optimization. The OneTaq One-Step RT-PCR Kit is capable of amplifying long transcripts up to 9 kb in length.
New England Biolabs, Inc. Luna Universal One-Step RT-qPCR Kit – 200 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
Alkali Scientific Radiant™ Probe 1-Step Lo-Rox qPCR Virus Kit, 1000 x 20µl Reactions, 4X
Recommended kit for SARS-CoV-2 diagnostics Validated for qualitative detection of SARS-CoV-2 nucleic acid Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays Ultra-sensitive detection of RNA viruses Earlier detection of a wide range of template amounts Includes UltraScript RTase for thermostable reverse transcription up to 55°C Advanced RNase inhibitor Antibody-mediated hot start technology Compatible with all real-time PCR platforms – standard and fast cycling conditions The kit has been optimised with a high-concentration 4x mix, enabling greater sample input and increasing sensitivity, even when working with small volume reactions.
Genscript Corporation High-Stability PCR Kit
GenScript High-Stability PCR Kit contains Green i Taq /i DNA polymerase 10 Taq buffer and Stabilized dNTP Mix 10 mM Each all specially formulated for increased stability using our proprietary technology The kit can be stored at 4 for over six months or at room temperature (25 ) for one month without significant loss of activity The stabilizers in the buffers also increase the stability of Green Taq DNA polymerase at higher temperature (72 ) increasing the DNA yield of PCR amplifications of long DNA This kit facilitates quick and easy PCR setup without any need for thawing This kit can also be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR setup at any time
ABclonal Technology Lyophilization One Step RT-qPCR Probe Kit
The Lyophilization One-Step RT-qPCR Probe Kit is a ready-to-use, freeze-dried reagent for probe-based RT-qPCR, compatible with lyophilized powder or microspheres. It integrates ABScript III Reverse Transcriptase, hot-start Taq polymerase, heat-labile UDG, and RNase inhibitor for efficient one-tube reactions, boosting throughput. The dUTP/UDG system prevents contamination, with UDG degrading at 50°C to avoid interference. Stable at 45°C for 6 months, it contains <0.2% glycerol and lyoprotectants for long-term performance.