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Standard PCR Products

Direct PCR Reagents and Kits (11)
Standard PCR Reagents and Kits (4)

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1

Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
GREENER_CHOICE

The TaqMan™ Cells-to-CT™ Express Kit enables rapid, reproducible gene expression analysis directly from cultured cells—allowing you to skip RNA extraction entirely. By removing extraction steps, the workflow reduces sample loss, shortens time to results, and improves consistency across experiments and operators.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Frozen, Dry Ice
Target cDNA
Content And Storage Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C)
Format Kit
Sample Type Mammalian Cells
Isolation Technology Direct cell lysis
Kit Type Direct Cell Lysis RT-qPCR
Purification Target RNA
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
High-throughput Compatibility Yes
PCR Method 2-step RT-qPCR
Detection Method TaqMan
2
Promotions Available

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit
GREENER_CHOICE

The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Sample Type Mammalian Cells
Reaction Speed 7 min.
Purification Time 7 min.
Starting Material 1-10 Cells
For Use With (Application) RT-PCR
PCR Method 2-step RT-qPCR
Product Line Ambion, Cells-to-CT
Detection Method TaqMan
3

Invitrogen™ Cells-to-cDNA™ II Kit

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

EDGE ENCOMPASS_PREFERRED
Shipping Condition Dry Ice
Content And Storage 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at
-20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase M-MLV
Size (Final Product) 7 kb or less
Final Product Type cDNA
Sample Type Mammalian Cells
Purification Time 2 hr.
Optimal Reaction Temperature 42°C
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Product Line Ambion, Cells-to-cDNA
4

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

ENCOMPASS_PREFERRED
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) RT-PCR
Product Line CellsDirect, Platinum, SuperScript
Detection Method Primer-probe
5

Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

ENCOMPASS_PREFERRED
For Use With (Equipment) 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cells
Reaction Speed Fast or Standard
Concentration 2X
For Use With (Application) RT-PCR
Product Line CellsDirect, Platinum, SuperScript
Detection Method Primer-probe
6

Thermo Scientific™ ThermoPrime Taq DNA Polymerase, with 10X buffer and separate vial of 25 mM MgCl2

Thermo Scientific ThermoPrime Taq Taq Polymerase is a 94 kDa recombinant thermostable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in E. coli.

7

Promega GoScript™ Reverse Transcription System

GoScript Reverse Transcriptase utilizes M-MLV and state-of-the-art buffer technology designed for qPCR to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibitors.

ENCOMPASS
For Use With (Application) RT-PCR,Real Time PCR (qPCR)
8

Biotium EvaEZ™ Fluorometric DNA Polymerase Activity Assay (200 rxn)

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes. It contains EvaGreen dye together with a primed template, dNTPs, and MgCl2 in a Tris buffer system. In the presence of DNA polymerase activity, the primer will be extended to form a double stranded product that can bind the EvaGreen dye, resulting in an increase of fluorescence. The rate of increase of fluorescence is positively correlated to the activity of the polymerase. The assay was developed for the measurement of Taq DNA polymerase activity. It can also be used for other DNA polymerases such as Pfu, Vent, Phusion, Bst, Phi29, MMLV, AMV, SuperScript, T4 DNA polymerase, T7 DNA polymerase, Klenow, and E. coli DNA polymerase I. The activity assay can be conducted at temperatures from 4°C to 75°C.

ENCOMPASS_PREFERRED