Lectins
Vector Laboratories Concanavalin A (ConA) Agarose Bound 10 ml
Concanavalin A (Con A) is one of the most widely used and well characterized lectins. Con A has broad applicability primarily because it recognizes a commonly occurring sugar structure, a-linked mannose. Agarose Bound Con A is prepared using affinity-purified lectins and heat stable, cross-linked 4% agarose beads. The attachment of the lectins to the beads through a hydrophilic spacer arm is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins. The recommended inhibiting/eluting sugar is a mixture of 200 mM a-methylmannoside/200 mM a-methylglucoside.
Vector Laboratories Sambucus Nigra Lectin (SNL, SNA, EBL) Agarose Bound 2ml
Sambucus nigra lectin (SNL) is isolated from elderberry bark and binds preferentially to sialic acid attached to terminal galactose in a-2,6 and to a lesser degree, a-2,3 linkage. Also called SNA (Sambucus nigra agglutinin) and EBL (elderberry lectin), SNL can inhibit cell-free protein synthesis. This agarose-bound SNL is prepared using affinity-purified lectin and heat stable, cross-linked 4% agarose beads. The attachment of the lectin through a hydrophilic spacer arm to the beads is carefully controlled to preserve the activity of and minimize conformational changes to the bound lectins. The recommended inhibiting/eluting sugar is 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid.
Vector Laboratories GSL I - Isolectin B4 (GSL I-B4 , BSL I-B4) Fluorescein-labeled 0.5 mg
Griffonia simplicifolia lectin I (GSL I) is a family of glycoproteins with molecular weights of approximately 114 kDa. Two subunits ("A" and "B") have slightly different molecular weights and combine to form tetrameric structures, resulting in five isolectins. The "A"-rich lectin preferentially agglutinates blood group A erythrocytes and appears to be specific for α-N-acetylgalactosamine residues, while the "B"-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins and binds several glycoproteins, including laminin. This fluorescein-labeled GSL I features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide.
Vector Laboratories Concanavalin A (ConA) Biotinylated 5 mg
Concanavalin A (ConA) recognizes α-linked mannose residues, which are present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. This biotinylated ConA conjugate features a ratio of biotin to lectin protein that provides optimal avidin/streptavidin and lectin binding activity. Supplied essentially free of unconjugated biotins, it is preserved with sodium azide.
Vector Laboratories Vicia Villosa Lectin (VVL, VVA), Fluorescein 2mg
VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.Fluorescein labeled Vicia villosa lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Vector Laboratories Maackia Amurensis Lectin I (MAL I), Unconjugated, 5mg
Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.