Ilex Life Sciences FGF-1 (FGF-acidic) Rat, E. coli Recombinant Protein (1 mg). DESCRIPTION: Our bioactive FGF-1 Rat, E. coli Recombinant Protein, also known as Fibroblast Growth Factor 1, is a single, non-glycosylated, polypeptide chain that contains 141 amino acids and has a 15.9 kDa molecular mass. The FGF-1 is purified by proprietary chromatographic techniques. FORMULATION: Sterile filtered white lyophilized (freeze-dried) powder. The protein was lyophilized at a concentration of 1 mg/ml in 5mM Na2PO4, pH-7.5 and 50mM NaCl. PURITY: Greater than 98.0% (SDS-PAGE). BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2 ng/ml corresponding to a Specific Activity of 5x106 IU/mg. OTHER NAMES: Fibroblast Growth Factor-1, FGF1, FGF-1, FGF acidic, FGF-a, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1, UniProtKB# P61149.