Poly-L-ornithine hydrobromide (MW 30,000-70,000) is a positively charged polyamino acid used to coat cell-culture surfaces to enhance cell attachment and growth. It is commonly applied to promote adhesion of primary neurons and other cell types in in vitro studies. The material is a white to off-white solid, soluble in water (≈50 mg/mL with warming and ultrasonic treatment), and supplied in small laboratory quantities.
- Enhances cell adhesion on culture vessels.
- Supports primary neuronal cultures, including dopaminergic neurons and oligodendrocytes.
- Positively charged polymer provides a uniform coating.
- Water soluble (≈50 mg/mL) with ultrasonic treatment and warming.
- Supplied as a solid with a defined molecular weight range of 30,000-70,000 Da.