Cell Culture Media, Supplements, and Reagents
Corning™ CellStripper Dissociation Reagent
Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture
Gibco™ Expi293™ PRO Expression Medium
Expi293 PRO Expression Medium supports rapid, high-yield protein production from HEK293 cells. Designed for scalable, high-density cultures with consistent expression across a variety of proteins, including complex and low-yield targets.
Puromycin dihydrochloride, 10 mg/ml in distilled water, sterile-filtered
CAS: 58-58-2 Molecular Formula: C22H31Cl2N7O5 Molecular Weight (g/mol): 544.43 MDL Number: MFCD00150080 InChI Key: MKSVFGKWZLUTTO-MLYJQVMKNA-N PubChem CID: 439530 ChEBI: CHEBI:17939 SMILES: Cl.Cl.COC1=CC=C(C[C@H](N)C(=O)N[C@@H]2[C@@H](CO)O[C@H]([C@@H]2O)N2C=NC3=C(N=CN=C23)N(C)C)C=C1
Gibco™ Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix
Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix
Corning™ G418 Sulfate, 50mg/mL
Blocks polypeptide synthesis by hindering protein elongation. Corning™ G418 Sulfate is an aminoglycoside similar in structure to gentamicin, neomycin and kanamycin.
Carbenicillin disodium salt
CAS: 4800-94-6 Molecular Formula: C17H16N2Na2O6S Molecular Weight (g/mol): 422.36 MDL Number: MFCD00077683 InChI Key: RTYJTGSCYUUYAL-YCAHSCEMSA-L PubChem CID: 20933 ChEBI: CHEBI:34609 SMILES: [Na+].[Na+].CC1(C)S[C@@H]2[C@H](NC(=O)C(C([O-])=O)C3=CC=CC=C3)C(=O)N2[C@H]1C([O-])=O
Gibco™ MEM α, nucleosides, no ascorbic acid
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Gibco™ Viral Production Medium
Viral Production Medium is a chemically defined, serum-free, and protein-free medium for growth and transfection of Viral Production Cells 2.0.
Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium
Used to enzymatically release adherent cells from tissue culture plates for passaging.
Corning™ Cell Culture Media: Cell Culture Supplements
Used as a nutrient source in cell culture media. Cell Culture Media: Cell Culture Supplements are produced under the highest industry standards to ensure superior results.
Tetracycline
CAS: 60-54-8 Molecular Formula: C22H24N2O8 Molecular Weight (g/mol): 444.44 MDL Number: MFCD00151232 InChI Key: JYHCQVWYCGHXGP-BPPSBWQWSA-N PubChem CID: 54675776 SMILES: CN(C)[C@H]1[C@@H]2C[C@H]3C(=C(O)C4=C(O)C=CC=C4[C@@]3(C)O)C(=O)[C@]2(O)C(=O)\C(=C(\N)O)C1=O
Hygromycin B
CAS: 31282-04-9 Molecular Formula: C20H37N3O13 Molecular Weight (g/mol): 527.524 MDL Number: MFCD06795479 InChI Key: GRRNUXAQVGOGFE-BBMONYMYSA-N PubChem CID: 134129613 IUPAC Name: (3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol SMILES: CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N
Gibco™ Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix
A soluble form of basement membrane extracted from murine Engelbreth-Holm-Swarm (EHS) tumors. It is free of viruses, including lactose dehydrogenase elevating virus (LDEV), sometimes found in this type of preparation.