Additional Cell Culture Reagents and Supplements
Promocell GmbH PromoCell Detach Kit-125 3 x 125 ml, Contains: C-40010, C-41010, C-41110
The PromoCell DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells.The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.PromoCell DetachKit was designed for the safe and efficient detachment of primary human cells in routine subculturing. Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%.HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.
Alkali Scientific CellPro™ HBSS with calcium and magnesium, 500ml
Balanced salt solution for cell culture applications (liquid form). All formulations incorporate a rigorous specification and approval process. Products with the LS and FS extension are supplied in convenient HyQPAK six-packs. Buffer cell culture growth with CellPro™ 1X Balanced Salt Solutions, available in lot sizes that facilitate scale-up and manufacturing.
ILEX LIFE SCIENCES LLC PAN Biotech DMSO Dimethyl Sulfoxide Solution for cell culture 100 ML 1/EA
PAN-Biotech Dimethyl Sulfoxide (DMSO) for Cell Culture 100 ml DESCRIPTION PAN-Biotech Dimethyl Sulfoxide (DMSO) solution for cell culture applications SUMMARY Dimethylsulfoxide (DMSO) is a colorless organic solvent which enters the cell and distributes inside the cell to prevent the formation of damaging ice crystals during the freezing procedure CAS REGISTRY NO 67-68-5 ENDOTOXIN TESTED No An endotoxin-tested version is also available (cat no P60-36750100) LIQUID/POWDER Liquid PRODUCT CATEGORY Media supplements STERILE No STORAGE Room temperature (RT) MANUFACTURER PAN-Biotech GmbH COUNTRY OF ORIGIN Germany
ILEX LIFE SCIENCES LLC PAN Biotech DMSO Dimethyl Sulfoxide Solution for cell culture endotoxin tested 100 ML 1/EA
PAN-Biotech Dimethyl Sulfoxide (DMSO) for Cell Culture Endotoxin Tested 100 ml DESCRIPTION PAN-Biotech Dimethyl Sulfoxide (DMSO) solution for cell culture applications Endotoxin tested SUMMARY Dimethylsulfoxide (DMSO) is a colorless organic solvent which enters the cell and distributes inside the cell to prevent the formation of damaging ice crystals during the freezing procedure CAS REGISTRY NO 67-68-5 ENDOTOXIN TESTED Yes LIQUID/POWDER Liquid PRODUCT CATEGORY Media supplements STERILE No STORAGE Room temperature (RT) MANUFACTURER PAN-Biotech GmbH COUNTRY OF ORIGIN Germany
Mabtech Inc Mouse Memory B-Cell Stimpack
Stimulation of B cells for use in mouse IgA, IgG and IgM ELISpot and FluoroSpot.
Sigma Aldrich Fine Chemicals Biosciences Lipopolysaccharides from Escherichia coli O111:B4 -irradiated, BioXtra, suitable for cell culture, MFCD00164401
Lipopolysaccharides from Escherichia coli O111:B4 -irradiated, BioXtra, suitable for cell culture
Sigma Aldrich Fine Chemicals Biosciences Nicotinamide | 98-92-0 | MFCD00006395 | 100g
Nicotinamide | Purity: ≥98% (HPLC), powder | MW:122.12 | 98-92-0 | MFCD00006395 | 100g
Promocell GmbH PromoCell CaCl 2-Solution 2 ml, 0.5 M
Some types of primary human cells, especially Normal Human Epidermal Keratinocytes (NHEK), respond sensitively to the calcium concentration in the culture medium. Calcium Chloride solution allows the adjustment of the optimal amount of calcium required by the cells. PromoCell Calcium Chloride (CaCl~2~) Solution is prepared as a solution from highly pure tissue culture tested Calcium Chloride and cell culture-grade water. It is a clear colorless and sterile solution.The concentration of CaCl~2~ is 0.5 M.
Promocell GmbH PromoCell Supplement Mix / Osteoblast Growth Medium
PromoCell Osteoblast Growth Medium has been optimized for the in vitro cultivation of human osteoblasts. The fetal calf serum in the medium is strictly pretested for optimal performance.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* SupplementMix: Contains all media supplements premixed in one vial| Media supplement | Final supplement concentration|| Fetal Calf Serum | 0.1 ml / ml |
PLANT CELL TECHNOLOGY RPMI-1640 Medium with L-Glutamine and 25mM HEPES, Cell Culture Media, Mod. 9, Clear Red-Orange Liquid, Sterile, 500mL bottle.
RPMI-1640 Mod. 9 is a sterile basal medium formulated for mammalian cell culture, supporting growth of lymphoid and hematopoietic cells as well as adherent lines. Supplied in a clear 500mL bottle, ready-to-use and stable under recommended conditions. Ideal for research labs, tissue engineering, and biopharmaceutical development workflows.
Promocell GmbH PromoCell Supplement Pack / Endothelial Cell Growth Medium MV 2 Contains: C-37310, C-30224, C-31063, C-30260, C-30321, C-31700, C-31750
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Quality Biological Inc Sodium Bicarbonate Solution, 100 mL
Sodium Bicarbonate (NaHCO3) is typically used in cell culture media as a buffer. ¿ Endotoxin and Mycoplasma Tested ¿ Although NaHCO3 does not have the buffering capacity of HEPES, it is occasionally preferred over HEPES because of its lower toxicity and nutritional value to cells in culture. ¿ Applications: Sodium Bicarbonate, 7.5% is useful as a buffer for culturing a broad spectrum of mammalian cells. ¿ Recommendation: dilute Sodium Bicarbonate in appropriate cell culture media, to a final concentration of 5-15 mM ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 290 ¿ 5% mOsM/kg H2O ¿ Concentration: 0.893 mol/L ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 24 months
Promocell GmbH PromoCell Supplement Pack / Fibroblast Growth Medium Contains: C-30310, C-31010
PromoCell Fibroblast Growth Medium is optimized for the cultivation of neonatal and juvenile fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vialsWhile the supplements of the Fibroblast Growth Medium are serum-free, the Basal Medium is manufactured with 0.5% serum.| Media supplement | Final supplement concentration|| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Supplement Mix / Endothelial Cell Growth Medium
Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.1 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Heparin | 90 μg / ml || Hydrocortisone | 1 μg / ml |Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Lifeline Cell Technology Fibroblast Supp. Kit
FibroLife S2 LifeFactors Kit. Supplements for culturing fibroblasts in a 2% FBS environment. Best paired with LM-0001 FibroLife Basal Medium.