Additional Cell Culture Reagents and Supplements
Sigma Aldrich Fine Chemicals Biosciences Methotrexate hydrate, 133073-73-1, MFCD00150847, 100 mg
Empirical Formula (Hill Notation): C20H22N8O5 · xH2O, Molecular Weight: 454.44 (anhydrous basis), powder, BioReagent, suitable for cell culture, ≥98% (HPLC), Synonym: 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N10-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate. Recommended for use in cell culture and molecular biology applications at 0.01-300 uM in nucleoside-free cell culture medium to select for DHFR expression.
Sigma Aldrich Fine Chemicals Biosciences ECM Gel from Engelbreth-Holm-Swarm murine sarcoma | MFCD00283131 | 1 mL
ECM Gel from Engelbreth-Holm-Swarm murine sarcoma | MFCD00283131 | 1 mL
AMERICAN RESEARCH PRODUCTS INC HEPES-BUFFERED TYRODE SOLUTION
NC0902546 HEPES-BUFFERED TYRODE SOLUTION
Promocell GmbH PromoCell Supplement Mix / Chondrocyte Growth Medium
PromoCell Chondrocyte Growth Medium has been developed for the in vitro cultivation of human chondrocytes. The fetal calf serum in the medium is strictly pretested for optimal performance.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for primary porcine and rat chondrocytes.To view a list of references where this medium was used with other species/cell types please check manufacturer homepage.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.1 ml / ml |
Promocell GmbH PromoCell Supplement Pack / Fibroblast Growth Medium Contains: C-30310, C-31010
PromoCell Fibroblast Growth Medium is optimized for the cultivation of neonatal and juvenile fibroblasts.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Components:* Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMix* Growth Medium Kit: Includes Basal Medium and SupplementPack* SupplementMix: Contains all media supplements premixed in one vial* SupplementPack: Contains all media supplements as individual vialsWhile the supplements of the Fibroblast Growth Medium are serum-free, the Basal Medium is manufactured with 0.5% serum.| Media supplement | Final supplement concentration|| Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Insulin (recombinant human) | 5 μg / ml |
Promocell GmbH PromoCell Supplement Pack / Endothelial Cell Growth Medium MV 2 Contains: C-37310, C-30224, C-31063, C-30260, C-30321, C-31700, C-31750
Endothelial Cell Growth Medium MV2 is a low-serum (5% V/V) medium developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.The medium lacks the Endothelial Cell Growth Supplement (ECGS, bovine hypothalamic extract) but contains basic Fibroblast Growth Factor, Insulin-like Growth Factor and Vascular Endothelial Growth Factor (VEGF). Because of its effects on cell metabolism, VEGF may interfere with certain experimental setups. In these cases, we recommend the use of Endothelial Cell Growth Medium MV.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.05 ml / ml || Epidermal Growth Factor (recombinant human) | 5 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 10 ng / ml || Insulin-like Growth Factor (Long R3 IGF) | 20 ng / ml || Vascular Endothelial Growth Factor 165 (recombinant human) | 0.5 ng / ml || Ascorbic Acid | 1 μg / ml || Hydrocortisone | 0.2 μg / ml |
Promocell GmbH PromoCell Hepes BSS (30 mM) 125 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).
Promocell GmbH PromoCell Detach Kit-125 3 x 125 ml, Contains: C-40010, C-41010, C-41110
The PromoCell DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells.The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.PromoCell DetachKit was designed for the safe and efficient detachment of primary human cells in routine subculturing. Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%.HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.
Promocell GmbH PromoCell Supplement Mix / Endothelial Cell Growth Medium
Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels.Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.| Media supplement | Final supplement concentration || Fetal Calf Serum | 0.02 ml / ml || Endothelial Cell Growth Supplement | 0.004 ml / ml || Epidermal Growth Factor (recombinant human) | 0.1 ng / ml || Basic Fibroblast Growth Factor (recombinant human) | 1 ng / ml || Heparin | 90 μg / ml || Hydrocortisone | 1 μg / ml |Note: For best transfection results we recommend using our heparin-free Endothelial Cell Growth Medium MV2.
Alkali Scientific CellPro™ HBSS with calcium and magnesium, 500ml
Balanced salt solution for cell culture applications (liquid form). All formulations incorporate a rigorous specification and approval process. Products with the LS and FS extension are supplied in convenient HyQPAK six-packs. Buffer cell culture growth with CellPro™ 1X Balanced Salt Solutions, available in lot sizes that facilitate scale-up and manufacturing.
STEMCELL Technologies StemSpan™ Myeloid Expansion Supplement II (100X), Size: 1 mL
StemSpan™ Myeloid Expansion Supplement II (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD14+ monocytes from CD34+ cells isolated from human cord blood (CB) samples.StemSpan™ Myeloid Expansion Supplement II (100X) is intended for use in combination with any of the following StemSpan™ media:• StemSpan™ SFEM (Catalog #09600)• StemSpan™ SFEM II (Catalog #09605)• StemSpan™-XF (Catalog #100-0073)• StemSpan™-AOF (Catalog #100-0130)When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement II typically promotes the production of hundreds of CD14+ monocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.