Additional Cell Culture Reagents and Supplements
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture | 6X1L
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture | 6X1L
U.S. Pharmacopeia Poloxalene, 9003-11-6, MFCD00082049, 500 mg
Mol wt: Mw ~3000, These products are for test and assay use only. They are not meant for administration to humans or animals and cannot be used to diagnose, treat, or cure diseases of any kind, Synonym: Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol).
Zymo Research Corporation Overexpression Broth (OB), 500 ml
Uniquely formulated Overexpression Broth™ (OB™) that is used in conjunction with our Expansion Broth™ (EB™) for consistent high level recombinant protein expression.
Stemculture LLC StemCultures LLC StemBeads FGF2 (Trial)
StemBeads FGF2 is a patented growth factor supplement that offers a novel way to culture cells with native Fibroblast Growth Factor 2 more efficiently. FGF2 is key for growth of human induced pluripotent stem cells (iPSCs), fibroblast cells, wound healing cells such as macrophages, cancer cells, and neural progenitor cells (NPCs). StemBeads FGF2 are microbeads composed of an FDA approved, biodegradable PLGA polymer that is loaded with native recombinant human FGF2. Controlled delivery and stable levels overcome the 4.5 hour half-life of FGF2 and improve cell cultures while saving researchers valuable time and resources. StemBeads FGF2 have been tested in media such as mTeSR, mTeSR plus, Stemflex, Flex 8, Nutristem, 10% FBS, and neuronal medium including neural progenitor expansion medium (NPEM) with enhanced cellular profiles. StemBeads FGF2 can be combined with other StemBeads varieties.
STEMCELL Technologies StemSpan™ CD34+ Expansion Supplement (10X), Size: 10 mL
StemSpan™ CD34+ Expansion Supplement (10X) contains a combination of recombinant human cytokines and other additives formulated to selectively promote the expansion of CD34+ cells isolated from human cord blood (CB) or bone marrow (BM) samples.StemSpan™ CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human cord blood (CB) cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. This expansion may be further increased with the addition of small molecules such as UM729. See data tab for more details. StemSpan™ CD34+ Expansion Supplement (10X) is intended for use in combination with any of the following StemSpan™ media:• StemSpan™ SFEM (Catalog #09600)• StemSpan™ SFEM II (Catalog #09605)• StemSpan™-XF (Catalog #100-0073)• StemSpan™-AOF (Catalog #100-0130)
BIOGEMS INTERNATIONAL INC ITSE (Insulin-Transferrin-Selenium-Ethanolamine) (100x) 10mL
Insulin-Transferrin-Selenium-Ethanolamine (ITSE) is a basal media supplement and can be used to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Insulin promotes the uptake of glucose and amino acids, transferrin is a non-toxic iron carrier, selenium is required for the function of glutathione peroxidase and other antioxidant enzymes, and ethanolamine is a phospholipid precursor. The components of the solution are manufactured animal-free and it is provided as a 100x sterile concentrate.
Sigma Aldrich Fine Chemicals Biosciences Potassium chloride | 7447-40-7 | MFCD00011360 | 250 g
Potassium chloride | Purity: 99% | Mol Wt: 74.55 | 7447-40-7 | MFCD00011360 | 250 g
Sigma Aldrich Fine Chemicals Biosciences Insulin-Transferrin-Sodium Selenite Supplement
Insulin-Transferrin-Sodium Selenite Supplement | 50mg
STEMCELL Technologies ImmunoCult™ Human CD3/CD28 T Cell Activator
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens.This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. 1. Antibody complexes bind to and cross-link CD3 and CD28 cell surface ligands, thereby providing the required signals for T cell activation. ImmunoCult™ Human CD3/CD28 T Cell Activator can be used on the Seahorse XF Analyzer to measure T cell activation response and is also available as part of the Agilent Seahorse XF Hu T Cell Activation Assay Kit.This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP. STEMCELL can work with you to qualify this reagent as an AM under an approved investigational new drug or clinical trial application.
STEMCELL Technologies ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator
Achieve robust activation and expansion of T cells in the absence of magnetic beads, feeder cells, or antigens. This product’s gentle activation stimulus ensures a high viability of activated T cells, which can be further expanded in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) or other media for culturing human T cells. ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator consists of soluble antibody complexes that bind to and cross-link CD3, CD28, and CD2 cell surface ligands, thereby providing the required primary and co-stimulatory signals for T cell activation. This product is designed for cell therapy research applications, but may be qualified for use as an ancillary material following the framework outlined in USP. STEMCELL can work with you to qualify this reagent as an AM under an approved investigational new drug or clinical trial application.
Research Products International Corp Bile Salts Number 3, 25 Grams
Bile Salts No. 3 is refined bile salt, comprising mainly of sodium cholate and sodium deoxycholate. It is used in media as a agent to isolate enteric organisms and coliforms. It is an excellent inhibitor of gram positive bacteria such as streptococci and staphylocci.
