Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.

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Gibco™ Macrophage-SFM (1X)

Macrophage-SFM (1X)

Pricing & Availability

Gibco™ Advanced DMEM

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	DMEM (Dulbecco's Modified Eagle Medium)
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Reduced Serum

Gibco™ Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Pricing & Availability
Specifications

Cell Line	Sp2, Vero, Raji, Daudi, and Jurkat
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Classification	Animal Origin
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Reduced Serum

Gibco™ MEM, high glucose, high sodium bicarbonate

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability

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Gibco™ CMRL Medium, no glutamine

CMRL medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's 'L'cells under serum-free conditions.

Pricing & Availability

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Gibco™ AmnioMAX™ C-100 Basal Medium

Part of kit used to prepare fully-supplemented Gibco AmnioMAX C-100 Complete Medium developed for short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Form	Liquid
Sterility	Sterile-filtered

Gibco™ RPMI 1640 Medium, no glutamine

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Glutamine,No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Leukemic Cells
Sterilization Method	Sterile-filtered
With Additives	Phenol Red
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ DMEM/F-12, powder

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
With Additives	High Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Form	Powder
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Serum Level	Standard Serum Supplementation

Gibco™ MEM, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells.

Pricing & Availability
Specifications

Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
With Additives	Low Glucose,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Form	Liquid
Without Additives	No Glutamine,No HEPES,No Sodium Pyruvate
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Shelf Life	24 Months From Date of Manufacture
Concentration	1 X
Sterility	Sterile-filtered
Product Line	Gibco
Serum Level	Standard Serum Supplementation
Cell Type	Primary Rat Astrocytes

100

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Gibco™ MEM (Temin's modification) (2X), no phenol red

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media.

Pricing & Availability

101

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Gibco™ MEM α, nucleosides, no phenol red

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES,No Phenol Red
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
Sterilization Method	Sterile-filtered
With Additives	Low Glucose,Glutamine,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides
Product Type	MEM α (Minimum Essential Medium α)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Standard Serum Supplementation

102

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Gibco™ RPMI 1640 Medium, no glucose

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Pricing & Availability

103

Gibco™ IMDM

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	Jurkat, COS-7, and macrophage cells
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No α-Thioglycerol,No 2-Mercaptoethanol
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate
Product Type	IMDM (Iscove's Modified Dulbecco's Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco

104

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered

105

Gibco™ DMEM, powder, low glucose, pyruvate

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, 293, Cos-7, and PC-12
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
With Additives	Low Glucose,Glutamine,Phenol Red,Sodium Pyruvate
Form	Powder
Without Additives	No HEPES,No Sodium Bicarbonate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Serum Level	Standard Serum Supplementation
Cell Type	Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

Classical and Basal Cell Culture Media

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Gibco™ Advanced DMEM Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced RPMI 1640 Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, high glucose, high sodium bicarbonate Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ CMRL Medium, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ AmnioMAX™ C-100 Basal Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ RPMI 1640 Medium, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM (Temin's modification) (2X), no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ RPMI 1640 Medium, no glucose Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ IMDM Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Advanced DMEM

Gibco™ Advanced RPMI 1640 Medium

Gibco™ MEM, high glucose, high sodium bicarbonate

Gibco™ CMRL Medium, no glutamine

Gibco™ AmnioMAX™ C-100 Basal Medium

Gibco™ RPMI 1640 Medium, no glutamine

Gibco™ MEM, no glutamine

Gibco™ MEM (Temin's modification) (2X), no phenol red

Gibco™ MEM α, nucleosides, no phenol red

Gibco™ RPMI 1640 Medium, no glucose

Gibco™ IMDM

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate