Classical and Basal Cell Culture Media
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Gibco™ RPMI 1640 Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.
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| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Cell Type | Leukemic Cells |
| Sterilization Method | Sterile-filtered |
| With Additives | Glutamine,Phenol Red |
| Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | Gibco |
| Serum Level | Standard Serum Supplementation |
Gibco™ VP-SFM AGT™ Medium
Gibco VP-SFM AGT Medium is a flexible-format medium that is equivalent to VP-SFM with the addition of Advanced Granulation Technology for simplified preparation.
| Cell Line | MDCK, VERO, HEp2, BHK-21, COS-7 |
|---|---|
| Included Antibiotics | No antibiotics |
| Product Type | Serum Free Medium (SFM) |
| Form | Powder (AGT) |
| Endotoxin Level | Low |
| Culture Type | Adherent Cell Culture |
| Product Line | Gibco |
| Serum Level | Serum-free |
Gibco™ BenchStable™ MEM
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Gibco Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
| Content And Storage | Store at room temperature. Protect from light. |
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Pyruvate |
| Manufacturing Quality | cGMP-compliant under the ISO 9001: 2015 standard |
| Green Features | ACT Ecolabel (Gold) |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| With Additives | Low Glucose,GlutaMAX,Phenol Red |
| Product Type | MEM (Minimum Essential Medium) |
| Classification | Animal Origin-free |
| Shelf Life | 12 Months From Date of Manufacture |
| Product Line | BenchStable |
| Serum Level | Standard Serum Supplementation |
Gibco™ DMEM, powder, high glucose, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | HeLa, 293, Cos-7, and PC-12 |
| Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
| Form | Powder |
| Without Additives | No HEPES,No Sodium Bicarbonate |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Sterility | Sterile-filtered |
| Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
| With Additives | High Glucose,Glutamine,Phenol Red,Sodium Pyruvate |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
| Classification | Animal Origin-free |
| Shelf Life | 36 Months From Date of Manufacture |
| Product Line | Gibco |
| Serum Level | Standard Serum Supplementation |
Gibco™ HepatoZYME-SFM
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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HepatoZYME-SFM
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Gibco™ Basal Medium Eagle (BME)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.
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Gibco™ DMEM/F-12, HEPES
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sterilization Method | Sterile-filtered |
|---|---|
| With Additives | High Glucose,Glutamine,HEPES,Phenol Red,Sodium Pyruvate |
| Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
| Form | Liquid |
| Classification | Animal Origin-free |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
Gibco™ Medium 199, HEPES
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
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Gibco™ LHC-8 Medium (1X), without gentamicin
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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LHC-8 Medium (1X), without gentamicin
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Gibco™ DMEM/F-12, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ FreeStyle™ F17 Expression Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Cell Line | HEK293 |
|---|---|
| Culture Environment | CO2 |
| Purity or Quality Grade | Qualified for Further Manufacturing |
| Form | Liquid |
| Without Additives | No Glutamine |
| Endotoxin Level | < 1 EU/mL |
| Concentration | 1 X |
| For Use With (Application) | Protein expression, viral vector production |
| Sterility | Sterile |
| Product Type | Expression Medium |
| Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
| Culture Type | Suspension Cell Culture |
| Product Line | Freestyle |
| Serum Level | Serum-free |
Gibco™ Leibovitz's L-15 Medium, GlutaMAX™ Supplement
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate.
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| Product Type | Leibovitz's L-15 Medium |
|---|---|
| Form | Liquid |
| Without Additives | No HEPES,No Sodium Bicarbonate,No Glucose |
| pH | 7.4 to 7.9 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 12 Months |
| Concentration | 1 X |
Gibco™ Opti-MEM™ Reduced Serum Medium, powder
Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
| Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 30 months from date of manufacture |
| With Additives | Glutamine,Phenol Red |
| Product Type | Opti-MEM I |
| Form | Powder |
| Classification | Animal Origin |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Shelf Life | 30 Months From Date of Manufacture |
| Sterility | Sterile-filtered |
| Product Line | Gibco, Opti-MEM |
| Cell Type | Primary Fibroblasts |
Gibco™ DMEM/F-12, powder, HEPES
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ MEM α, no nucleosides
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Sterilization Method | Sterile-filtered |
|---|---|
| Product Type | MEM α (Minimum Essential Medium α) |
| Form | Liquid |
| Classification | Animal Origin-free |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Concentration | 1 X |
| Sterility | Sterile-filtered |
| Product Line | Gibco |
| Serum Level | Standard Serum Supplementation |