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Thermo Scientific™ DreamTaq PCR Master Mixes (2X)

Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.

Shipping Condition | Dry Ice |
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Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
For Use With (Application) | Standard PCR |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | First-Strand cDNA |
Quantity | Each |
Concentration | 5X |
For Use With (Application) | Real Time PCR (qPCR) |
Detection Method | qPCR |
Format | Master Mix |
Size (Final Product) | Up to 10 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Optimal Reaction Temperature | 50°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
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Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
Thermo Scientific™ FastDigest DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Dpn I |
Methylation Sensitivity | Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | Blunt |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.

Product Type | T4 Polynucleotide Kinase |
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Enzyme | T4 Polynucleotide Kinase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 10 U/μL |
Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | EcoR I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest XhoI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Xho I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Product Type | T4 DNA Ligase |
---|---|
Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | Hot Start DNA Polymerase |
Reaction Speed | Fast or Standard |
Overhang | 3'-A |
Concentration | 2X |
Thermo Scientific™ FastDigest Esp3I (IIs class)

Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Product Type | Alkaline Phosphatase |
---|---|
Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentration | 1 U/μL |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.

Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Plus DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >100X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 sec./kb |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ FastDigest BamHI

Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BamH I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |