Life Science Research ELISA Kits

ELISA kits and systems for the quantification of target antigens in human, mouse, rat, bovine, and other tissue extracts, serums, and cell cultures; includes highly validated materials for accurate quantitation and long-term reproducibility.

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Invitrogen™ Cell Extraction Buffer

Suitable for use in ELISA and Western blotting

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Invitrogen™ Human IL-10 ELISA Kit

ELISA

Pricing & Availability
Specifications

Measurement	Absorbance at 450 nm minus absorbance (at 550 nm)
Immunoassay Kit Format	Colorimetric ELISA (TMB substrate) with Plates
Target Species	Human
Conjugate	HRP
Description	Human IL-10 ELISA Kit
Interassay CV	<10%
Intraassay CV	<10
Gene Symbol	CSIF,GVHDS,IL-10 A,TGIF,IL10
Total Assay Time	3 hrs
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 50 μL
Assay Sensitivity	<3 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.36 to 600 pg/mL
Test Time	1 hrs 20 mins
Accession Number	P22301
Regulatory Status	RUO
Target	IL-10
Specificity	No significant cross-reaction with human IL-1 A, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Precision	Intra-assay CV <10%; inter-assay CV <10%
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3586
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ Human GFAP ELISA Kit

ELISA

Pricing & Availability

Invitrogen™ Human Fibronectin ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	6.7%
Intraassay CV	5.3
Gene Symbol	CIG,ED-B,FINC,FN,FNZ,GFND,GFND2,LETS,MSF
Total Assay Time	3 hrs 10 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 50 μL, Urine 50 μL
Assay Sensitivity	0.1 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.31 to 20.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P02751
Regulatory Status	RUO
Target	Fibronectin
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	2335
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	HRP
Synonym	Cold insoluble globulin, FINC, LETS, MSF, FN

Invitrogen™ Mouse IgG1 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
Assay Sensitivity	3.13 ng/mL
Storage Requirements	2-8°C
Assay Range	3.13-200 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Regulatory Status	RUO
Target	Mouse IgG1
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin G1

Invitrogen™ Rat TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Rat
Conjugate	HRP
Interassay CV	10%
Intraassay CV	5%
Total Assay Time	3 hr 10 min
Sample Volume	Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	11.0 pg/mL
Storage Requirements	2-8°C
Assay Range	39.1-2500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P16599
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	24835
Sample Type	Serum,Supernatant
Label or Dye	Biotin
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

R&D Systems™ Human IL-6 Quantikine HS ELISA

Human IL-6 Quantikine HS ELISA Kit from the most referenced ELISA manufacturer. Highly validated for accurate quantitation and long-term reproducibility.

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Invitrogen™ Mouse IL-6 ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Aliases	IL-6, B-cell hybridoma growth factor, Interleukin HP-1
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	8.9%
Intraassay CV	5%
Gene Symbol	Il6
Total Assay Time	3 hr 30 min
Assay Sensitivity	6.5 pg/mL
Storage Requirements	2-8°C
Assay Range	31.3-2000 pg/mL
Test Time	1 hr 20 min
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6; IL-6
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	16193
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

Invitrogen™ Human Perforin ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	9.3%
Intraassay CV	4.9
Gene Symbol	PRF1
Total Assay Time	3 hrs 30 mins
Sample Volume	Cell Lysate 20 μL, Plasma 20 μL, Serum 20 μL
Assay Sensitivity	11.8 pg/ml
Storage Requirements	2°C to 8°C
Assay Range	28.1 to 1800 pg/mL
Test Time	1 hrs 20 mins
Accession Number	P14222
Regulatory Status	RUO
Target	Perforin
Kit Contents	Pre-coated 96 well plate,Standard (2 vials),Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,Streptavidin-HRP,Wash Buffer,Substrate Solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	5551
Sample Type	Cell Lysates,Plasma,Serum
Label or Dye	HRP

Invitrogen™ Human CD63 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10
Gene Symbol	CD63
Total Assay Time	4 hrs 45 mins
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	14 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	14 to 3,000 pg/mL
Test Time	1 hrs 20 mins
Accession Number	C75951; CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Cd63 molecule; CD63 protein; granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; mast cell antigen AD1; ME491; melanoma 1 antigen; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; TSPAN30; Tspan-30
Regulatory Status	RUO
Target	CD63
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	967
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

Invitrogen™ Human NFkB p65 (Total/Phospho) InstantOne™ ELISA Kit

ELISA

Pricing & Availability

Invitrogen™ Human Complement C3a ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Intraassay CV	5.9
Total Assay Time	4 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
Assay Range	0.3 to 20 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Regulatory Status	RUO
Target	Complement C3a
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	718
Sample Type	Plasma,Serum,Supernatant
Synonym	complement component 3, ASP, C3a, C3b, C3, Acylation stimulating protein

Invitrogen™ Human MCP-1 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	8.7%
Intraassay CV	4.7
Gene Symbol	GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF
Total Assay Time	2 hrs 10 mins
Sample Volume	Amniotic Fluid 20 μL, Plasma 20 μL, Serum 20 μL, Supernatant 20 μL
Assay Sensitivity	2.3 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.6 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P13500
Regulatory Status	RUO
Target	CCL2 (MCP-1)
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	6347
Sample Type	Amniotic Fluid,Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Monocyte chemoattractant protein 1, MCAF, HC11

Invitrogen™ Human IL-8 Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P10145
Regulatory Status	RUO
Target	IL-8
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3576
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-8, CXCL8, NAP-I

Invitrogen™ Mouse IgA Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.39 ng/mL
Storage Requirements	2°C to 8°C
Assay Range	0.39 to 25 ng/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Regulatory Status	RUO
Target	Mouse IgA
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	238447
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin A

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