Biological Buffers

Thermo Scientific™ Pierce™ Fast Blocking and Wash Buffers

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.

HEPES sodium salt, 99+%, for molecular biology, DNAse, RNAse and Protease free, ACROS Organics™

Chemical Name or Material: HEPES sodium salt Name Note: 99+% CAS: 75277-39-3 Purity Grade: For molecular biology Assay: ≥99.0% (on dry substance) Assay Percent Range: 99+% Molecular Formula: C8H17N2NaO4S Formula Weight: 260.27 MDL Number: MFCD00036463 Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt

Thermo Scientific™ Pierce™ StartingBlock™ Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS formulations of a special protein blocker for exceptional nonspecific blocking and broad compatibility.

MES sodium salt, 99%, ACROS Organics™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: MES sodium salt CAS: 71119-23-8 Assay: ≥98.5% (after ion exchange) Assay Percent Range: 99% Molecular Formula: C6H12NNaO4S Formula Weight: 217.22 MDL Number: MFCD00065473

ACES, 99%, extra pure, ACROS Organics™

Chemical Name or Material: ACES Name Note: Extra Pure CAS: 7365-82-4 CAS Min %: 98.5 CAS Max %: 100.0 Purity Grade: Extra Pure Assay: ≥98.5% Assay Percent Range: 99% Molecular Formula: C4H10N2O4S Linear Formula: H2NCOCH2NHCH2CH2SO3H Formula Weight: 182.19 MDL Number: MFCD00008030 Synonym: N-(2-Acetamido)-2-aminoethanesulfonic acid

HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free, ACROS Organics™

Chemical Name or Material: HEPES free acid CAS: 7365-45-9 Purity Grade: For molecular biology Assay: ≥99.0% (on dry substance) Assay Percent Range: 99+% Molecular Formula: C8H18N2O4S Formula Weight: 238.3 MDL Number: MFCD00006158 Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid

Thermo Scientific™ Phusion™ Buffers Available on GSA/VA Contract for Federal Government customers only.

Buffer packs for use in PCR with Phusion High-Fidelity DNA Polymerases

Thermo Scientific™ Phusion™ Buffers Available on GSA/VA Contract for Federal Government customers only.

Buffer packs for use in PCR with Phusion High-Fidelity DNA Polymerases

Thermo Scientific™ SuperBlock™ Blocking Buffers

Block membranes, plates and tissue samples with these optimized solutions containing a protein that provides fast and effective blocking for Western blot, ELISA and IHC.

HEPES sodium salt, 99%, ACROS Organics™

Chemical Name or Material: HEPES sodium salt Name Note: 99% CAS: 75277-39-3 Assay: ≥98.5% (on anhydrous substance) Assay Percent Range: 99% Molecular Formula: C8H17N2NaO4S Formula Weight: 260.27 MDL Number: MFCD00036463 Synonym: 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt

Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

PIPES, disodium salt, 99%, ACROS Organics™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: PIPES, disodium salt Assay: 98.5 to 101.5% (dry basis, acidimetry) Assay Percent Range: 99% Molecular Formula: C8H16N2Na2O6S2 Formula Weight: 346.33 Synonym: 1, 4-Piperazinediethanesulfonic acid disodium salt

ACES, 99+%, for biochemistry, ACROS Organics™

Chemical Name or Material: ACES CAS: 7365-82-4 CAS Min %: 99.0 CAS Max %: 100.0 Purity Grade: For biochemistry Assay: ≥99.0% Assay Percent Range: 99+% Molecular Formula: C4H10N2O4S Linear Formula: H2NCOCH2NHCH2CH2SO3H Formula Weight: 182.19 MDL Number: MFCD00008030 Synonym: N-(2-Acetamido)-2-aminoethanesulfonic acid

HEPES, 99%, for biochemistry, ACROS Organics™

Chemical Name or Material: HEPES CAS: 7365-45-9 Purity Grade: For biochemistry Assay: ≥98.5% (on dried substance) (Acid) Assay Percent Range: 99% Molecular Formula: C8H18N2O4S Formula Weight: 238.3

ADA, 99%, for biochemistry, ACROS Organics™

Chemical Name or Material: ADA Name Note: For biochemistry CAS: 26239-55-4 CAS Min %: 98.5 CAS Max %: 100.0 Purity Grade: For biochemistry Assay: ≥98.5% Assay Percent Range: 99% Molecular Formula: C6H10N2O5 Linear Formula: H2NCOCH2N(CH2CO2H)2 Formula Weight: 190.16 MDL Number: MFCD00008031 Synonym: N-(2-Acetamido)iminodiacetic acid

Thermo Scientific™ Pierce™ Protein-Free Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS and TBS formulations of a non-protein compound to yield extremely low background.

MES hydrate, 99%, for biochemistry, ACROS Organics™

Chemical Name or Material: MES hydrate Name Note: 99% CAS: 1266615-59-1 Purity Grade: For biochemistry Assay: ≥99.0% (on dry substance) (Acid) Assay Percent Range: 99+% Molecular Formula: C6H13NO4S·xH2O Formula Weight: 195.23 MDL Number: MFCD00149409 Synonym: 4-Morpholinoethanesulfonic acid hydrate

Thermo Scientific™ Phire Buffers Available on GSA/VA Contract for Federal Government customers only.

Choose from three formats of these buffers to optimize PCR using Thermo Scientific™ Phire™ Hot Start II DNA Polymerase.

Alfa Aesar™ Kinase buffer I (5X)

For cellular and molecular biology applications

Alfa Aesar™ Phosphatase buffer 1 (5X)

For cellular and molecular biology applications

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Tricine, 99+%, for biochemistry, ACROS Organics™ Available on GSA/VA Contract for Federal Government customers only.

Chemical Name or Material: Tricine CAS: 1389475 Purity Grade: For biochemistry Assay: ≥99% Assay Percent Range: 99+% Molecular Formula: C6H13NO5 Linear Formula: (HOCH)3CNHCHCOH Formula Weight: 179.17 MDL Number: MFCD00004277 Synonym: N-(2-Hydroxy-1, 1-bis(hydroxymethyl)ethyl)glycine

PIPES, 98.5+%, for biochemistry, ACROS Organics™

Chemical Name or Material: PIPES CAS: 5625-37-6 Purity Grade: For biochemistry Assay: ≥98.50% Assay Percent Range: 98.5+% Molecular Formula: C8H18N2O6S2 Formula Weight: 302.35 Synonym: 1, 4-Piperazinebis(ethanesulfonic acid)

Thermo Scientific™ Pierce™ Clear Milk Blocking Buffer

Thermo Scientific™ Pierce Clear Milk Blocking Buffer is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

Thermo Scientific™ Pierce™ LDS 4X Nonreducing Sample Buffer

Nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

Thermo Scientific™ Blocker™ Casein Blocking Buffers

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ Phusion Buffers Available on GSA/VA Contract for Federal Government customers only.

Choose between two buffer packs to optimize high-fidelity PCR or difficult or long template amplification using Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases.

Thermo Scientific™ Pierce™ Lane Marker Sample Buffers

Ready-to-use 5X SDS-PAGE sample loading buffers available in reducing and nonreducing formulations, both with a pink dye buffer-front marker.

Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

MES Monohydrate, 98%, Alfa Aesar™

Chemical Name or Material: MES Monohydrate Assay Percent Range: 98% Molecular Formula: C6H13NO4S·H2O Formula Weight: 213.26 (195.24 Anhydrous)

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