Protein Modification Reagents

Thermo Scientific™ 8M Guanidine-HCl Solution

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Traut's Reagent (2-iminothiolane)

2-Iminothiolane-HCl (CAS 4781-83-3); an amine-reactive thiolation reagent to cap protein primary amines with sulfhydryl groups for immediate crosslinking.

Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Thermo Scientific™ Pierce™ Iodoacetamide, Single-Use

Thermo Scientific™ Pierce™ Iodoacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization and peptide mapping.

Thermo Scientific™ Guanidine-HCl

Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format Available on GSA/VA Contract for Federal Government customers only.

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction System 1 Available on GSA/VA Contract for Federal Government customers only.

Induces protein expression without monitoring cell density.

Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this disulfide reducing agent.

Thermo Scientific™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Thermo Scientific™ Pierce™ Iodoacetic Acid

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)

Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ MS(PEG)24 Methyl-PEG-NHS-Ester Reagent

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.

Thermo Scientific™ Pierce™ Hydroxylamine-HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Thermo Scientific™ Pierce™ MMTS (methyl methanethiosulfonate)

Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.

Thermo Scientific™ Pierce™ Asp-N Protease, MS Grade

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ Pierce™ Chymotrypsin Protease (TLCK treated), MS Grade

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ EMCA (N-ε-maleimidocaproic acid)

Permanently cap protein or peptide cysteines with carboxyl groups (make caproic acid derivatives) using this sulfhydryl-reactive (maleimide) compound.

Thermo Scientific™ Pierce™ Chloroacetamide, No-Weigh™ Format

Thermo Scientific™ Pierce™ Chloroacetamide No-Weigh™ Format is a sulfhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterization.

Thermo Scientific™ Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)

Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.

Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL) Available on GSA/VA Contract for Federal Government customers only.

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ 2-Mercaptoethanol

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

Thermo Scientific™ MS(PEG)4 Methyl-PEG-NHS-Ester Reagent

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ Pierce™ Sodium meta-Periodate

Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).

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