Proteases and Protein-Cleaving Reagents

Thermo Scientific™ Pierce™ Proteases for Mass Spectrometry Available on GSA/VA Contract for Federal Government customers only.

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Asp-N Protease, MS Grade

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ Chymotrypsin Protease (TLCK treated), MS Grade

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/µL) Available on GSA/VA Contract for Federal Government customers only.

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Thermo Scientific™ Pierce™ Glu-C Protease, MS Grade

Improve sequence coverage and protein identification for mass spectrometry using these individual, highly specific proteases in custom combinations.

Thermo Scientific™ Immobilized Papain (Agarose Resin) Available on GSA/VA Contract for Federal Government customers only.

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Promega IdeS Protease

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.

Promega IdeZ Protease, 5,000 units

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.

Thermo Scientific™ Trypsin, TPCK Treated

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ DSSO (disuccinimidyl sulfoxide)

MS-cleavable and membrane-permeable crosslinker that contains amine-reactive N-hydroxysuccinimide (NHS) ester at each end of a 7-carbon spacer arm.

Promega AccuMAP Low pH Protein Digestion Mini Kit, 1 each

AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.

Promega AccuMAP Low pH Resistant rLys-C Solution, 300microl

AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.

Promega AccuMAP Denaturing Solution, 1ml

AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.

Thermo Scientific™ Proteinase K Powder (≥30 U/mg) Available on GSA/VA Contract for Federal Government customers only.

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

Thermo Scientific™ In-Gel Tryptic Digestion Kit

Trypsin-digest and recover peptide fragments for mass spectrometry from 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).

Affymetrix/USB™ Pepsin from Porcine Stomach

Derived from Porcine stomach

MilliporeSigma™ Proteinase K, Lyophilized Available on GSA/VA Contract for Federal Government customers only.

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

Gibco™ PNGase F Glycan Cleavage Kit Non-distribution product as customer accommodation.

PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.

MilliporeSigma™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides

MilliporeSigma™ Proteinase K Solution, 600mAU/mL

Efficient removal of proteins from nucleic acid solutions

Promega ProTEV Plus Protease

ProTEV Plus is an improved 48kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity.

Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)

Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.

Thermo Scientific™ Staphylococcus aureus V-8 Protease Available on GSA/VA Contract for Federal Government customers only.

Specifically cleave only at glutamic acid residues or cleave at both glutamic and aspartic residues with our immobilized Staphylococcus aureus V-8 protease kit.

Thermo Scientific™ In-Solution Tryptic Digestion and Guanidination Kit

Alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry (MS) using proteomics-grade trypsin, buffers and reagents.

MilliporeSigma™ Endopeptidase, Neutral, Porcine Kidney

Peptidase activity is specifically directed toward cleavage on amino side of hydrophobic residues

Promega Human Recombinant Tryptase

For use in identifying tryptase inhibitors and biological role in conjunction with mast cells in asthma, allergy and inflammation.

MilliporeSigma™ HRV 3C Protease

Highly efficient fusion tag removal at low temperature

Thermo Scientific™ Immobilized Ficin (Agarose Resin)

Enable controlled antibody fragmentation, especially of mouse IgG1, with this sulfhydryl-specific ficin protease immobilized onto beaded agarose resin.

Promega Iodoacetamide, 15mg

AccuMAP Low pH Protein Digestion Kit enables suppression of sample preparation-induced artificial deamidation, disulfide bond scrambling and oxidation overthe course of sample preparation. Pre-existing non-enzymatic modifications remain intact.

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