Modifying Enzyemes

Affymetrix/USB™ Proteinase K Lyophilized Powder

Proteinase K highly active and stable endopeptidase used in wide range of applications purified from fungus T. album.

Hexokinase, yeast, Alfa Aesar™

Used to catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing, ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.

Thermo Scientific™ Klenow Fragment Promotion

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Peroxidase, horseradish, Alfa Aesar™

Used in the oxidation of luminol to 3-aminophthalate. It is often used in conjugates to determine the presence of a molecular target, especially in the detection of specific protein in a western blot after conjugation with antibody.

Thermo Scientific™ DNA Polymerase I Available on GSA/VA Contract for Federal Government customers only.

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Ribonuclease A, bovine pancreas, Molecular Biology Grade, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

Thermo Scientific™ Klenow Fragment Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Deoxyribonuclease I, bovine pancreas, Purified, Alfa Aesar™

Used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.

Thermo Scientific™ EpiJET DNA Methylation Analysis Kit (MspI/HpaII)

Perform fast and efficient analysis of the DNA methylation status at a specific locus with the Thermo Scientific™ EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII).

Catalase Products for Science Education

Oxidizing Enzyme

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase Promotion

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Thermo Scientific™ T4 β-glucosyltransferase

Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis

Deoxyribonuclease I, bovine pancreas, Alfa Aesar™

Used for the removal of DNA from protein samples.

Thermo Scientific™ Agarase Available on GSA/VA Contract for Federal Government customers only.

Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.

Affymetrix/USB™ Phosphodiesterase I

Extracted from Crotalus adamanteus venom.

Thermo Scientific™ Endonuclease IV, E.coli (Endo IV) Available on GSA/VA Contract for Federal Government customers only.

Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.

Affymetrix/USB™ Diaphorase from Clostridium kluyveri

Used to study protein-protein interactions

Thermo Scientific™ Klenow Fragment, exo- Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Thermo Scientific™ EpiJET™ DNA Methylation Analysis Kit (TaqI/HpyF30I)

The EpiJET DNA Methylation Analysis Kit (TaqI/HpyF30I) uses TaqI and HpyF30I for fast and efficient analysis of the DNA methylation status at a specific locus.

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase Available on GSA/VA Contract for Federal Government customers only.

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Affymetrix/USB™ Pancreatin 1X

Extracted from porcine pancreas.

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