Modifying Enzyemes

Thermo Scientific™ Exonuclease III (Exo III) Available on GSA/VA Contract for Federal Government customers only.

Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.

Thermo Scientific™ RNase H Available on GSA/VA Contract for Federal Government customers only.

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

Invertase Products for Science Education

CAS: 9001-57-4

Thermo Scientific™ DNA Polymerase I Available on GSA/VA Contract for Federal Government customers only.

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Thermo Scientific™ RapidOut DNA Removal Kit

New proprietary technology for convenient removal of gDNA from RNA sample in a simple two-step procedure.

Pectinase, Aspergillus niger, Alfa Aesar™

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

Thermo Scientific™ S1 Nuclease Available on GSA/VA Contract for Federal Government customers only.

Degrade single-stranded DNA and RNA with this endonuclease.

Thermo Scientific™ Pyrophosphatase, Inorganic (from yeast) Available on GSA/VA Contract for Federal Government customers only.

Catalyze the hydrolysis of inorganic pyrophosphate to two orthophosphates.

Thermo Scientific™ Endonuclease IV, E.coli (Endo IV) Available on GSA/VA Contract for Federal Government customers only.

Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.

Thermo Scientific™ T4 DNA Ligase Available on GSA/VA Contract for Federal Government customers only.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Affymetrix/USB™ Proteinase K Lyophilized Powder

Proteinase K highly active and stable endopeptidase used in wide range of applications purified from fungus T. album.

Thermo Scientific™ DNase I, RNase-free Available on GSA/VA Contract for Federal Government customers only.

An endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Thermo Scientific™ T4 β-glucosyltransferase

Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase Available on GSA/VA Contract for Federal Government customers only.

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase Promotion

Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .

Thermo Scientific™ RNase I Available on GSA/VA Contract for Federal Government customers only.

Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.

Thermo Scientific™ Klenow Fragment Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Ribonuclease A, bovine pancreas, purified, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

Affymetrix/USB™ Phosphodiesterase I

Extracted from Crotalus adamanteus venom.

Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) Available on GSA/VA Contract for Federal Government customers only.

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.

Thermo Scientific™ RiboLock RNase Inhibitor Available on GSA/VA Contract for Federal Government customers only.

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

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