Modifying Enzyemes

Thermo Scientific™ Klenow Fragment, exo- Promotion

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Thermo Scientific™ EpiJET DNA Methylation Analysis Kit (MspI/HpaII)

Perform fast and efficient analysis of the DNA methylation status at a specific locus with the Thermo Scientific™ EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII).

Thermo Scientific™ EpiJET™ DNA Methylation Analysis Kit (TaqI/HpyF30I)

The EpiJET DNA Methylation Analysis Kit (TaqI/HpyF30I) uses TaqI and HpyF30I for fast and efficient analysis of the DNA methylation status at a specific locus.

Lipase, from porcine pancreas, Alfa Aesar™

Used as a catalyst in the preparation of biodiesels and in the preparation of esters of chiral epoxy alcohols. Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Thermo Scientific™ RNase H Available on GSA/VA Contract for Federal Government customers only.

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

Thermo Scientific™ Klenow Fragment Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Thermo Scientific™ T4 DNA Ligase Available on GSA/VA Contract for Federal Government customers only.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Hexokinase, yeast, Alfa Aesar™

Used to catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing, ATP as a phosphate source. Used to determine glucose, fructose, mannose and ATP.

Thermo Scientific™ Proteinase K (recombinant), PCR grade Available on GSA/VA Contract for Federal Government customers only.

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Thermo Scientific™ S1 Nuclease Available on GSA/VA Contract for Federal Government customers only.

Degrade single-stranded DNA and RNA with this endonuclease.

Thermo Scientific™ Klenow Fragment Promotion

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Casein hydrolysate enzymatic, Alfa Aesar™

Used as nutritional source in microbial media. It is derived from the enzymatic digest of casein, often used in culture media. NZ Amine EKC contains a mixture of peptides and amino acids.

Affymetrix/USB™ Cellulase from Aspergillus niger

Cellulolytic enzyme isolated from Aspergillus niger

Thermo Scientific™ Exonuclease I (Exo I) Available on GSA/VA Contract for Federal Government customers only.

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Ascorbate oxidase, from Cucurbita sp., Alfa Aesar™

May be used to study oxidative stress and heat stress response and tolerance.

Thermo Scientific™ Endonuclease V, T.maritima (Endo V) Available on GSA/VA Contract for Federal Government customers only.

Degrade DNA with Endonuclease V, T. maritima (Endo V), a 3'-endonuclease involved in DNA repair that initiates removal of deaminated bases from damaged DNA.

Thermo Scientific™ T4 DNA Ligase Promotion

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Deoxyribonuclease I, bovine pancreas, Alfa Aesar™

Used for the removal of DNA from protein samples.

Deoxyribonuclease I, bovine pancreas, Purified, Alfa Aesar™

Used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.

Pectinase, Aspergillus niger, Alfa Aesar™

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

Thermo Scientific™ DNA Polymerase I Available on GSA/VA Contract for Federal Government customers only.

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

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