Modifying Enzyemes

Thermo Scientific™ Proteinase K (recombinant), PCR grade Available on GSA/VA Contract for Federal Government customers only.

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Thermo Scientific™ T4 DNA Ligase Available on GSA/VA Contract for Federal Government customers only.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Thermo Scientific™ T4 β-glucosyltransferase

Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis

Thermo Scientific™ T4 DNA Ligase Promotion

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Thermo Scientific™ RiboLock RNase Inhibitor Available on GSA/VA Contract for Federal Government customers only.

RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.

Thermo Scientific™ Klenow Fragment Promotion

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Thermo Scientific™ RNase I Available on GSA/VA Contract for Federal Government customers only.

Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.

Deoxyribonuclease I, bovine pancreas, Alfa Aesar™

Used for the removal of DNA from protein samples.

Thermo Scientific™ T4 RNA Ligase Available on GSA/VA Contract for Federal Government customers only.

Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.

Affymetrix/USB™ Phosphodiesterase I

Extracted from Crotalus adamanteus venom.

Deoxyribonuclease I, bovine pancreas, Purified, Alfa Aesar™

Used in RNA preparations from tissue or bacterial cell cultures. Deoxyribonuclease is used to remove DNA from protein samples, It is also used in a study to compare several procedures for reducing RNase contamination in preparations of DNase.

Proteinase K, Tritirachium album limber, Alfa Aesar™

Commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Thermo Scientific™ EpiJET 5-hmC and 5-mC Analysis Kit

Get fast and reliable assessment of both cytosine modifications - 5-hmC and 5-mC - within CCGG loci

Thermo Scientific™ Agarase Available on GSA/VA Contract for Federal Government customers only.

Specifically digests the agarose polysaccharide core, which allows for gentle yet efficient recovery of DNA or RNA fragments from low melting point agarose.

Invertase Products for Science Education

CAS: 9001-57-4

Thermo Scientific™ Klenow Fragment Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Collagenase, Type I, Clostridium histolyticum, Alfa Aesar™

Used as a powder and solvent injection kit for the treatment of dupuytren's contracture.

Ribonuclease A, bovine pancreas, purified, Alfa Aesar™

Used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.

Affymetrix/USB™ Pancreatin 1X

Extracted from porcine pancreas.

Thermo Scientific™ DNA Polymerase I Available on GSA/VA Contract for Federal Government customers only.

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Thermo Scientific™ Exonuclease I (Exo I) Available on GSA/VA Contract for Federal Government customers only.

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

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