Reagents for Luciferase Reporter Assays

BD D- Luciferin Potassium Salt Available on GSA/VA Contract for Federal Government customers only.

Synthetically made substrate which can be catalyzed by firefly luciferase

Thermo Scientific™ TurboFect™ Cell Transfection Reagent

Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.

Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)

Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.

BD Monolight™ Luminometer Thermal Printer Paper Available on GSA/VA Contract for Federal Government customers only.

For recording data from luminometer instrument measurements

Thermo Scientific™ Pierce™ 100X Firefly Signal Enhancer

Produce maximum bioluminescence signal intensity and sensitivity with the Pierce Firefly Luciferase Flash and Glow Assays.

BD D- Luciferin Sodium Salt Available on GSA/VA Contract for Federal Government customers only.

Specific for firefly luciferase in the illustrated reactions

Promega™ Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System

Measures the chymotrypsin-like protease activity of the proteasome complex in cultured cells. Add the Proteasome-Glo Cell-Based Assay Reagent in an add-mix-measure format to produce proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

Promega™ VivoGlo™ Luciferin-β-Galactoside Substrate (6-O-β-galactopyranosyl luciferin)

Substrate for the commonly used reporter enzyme β-galactosidase. The substrate is cleaved by β-galactosidase to form luciferin and galactose. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light.

Promega™ Chroma-Glo™ Luciferase Assay System

Homogeneous assay1, 2, 3, 4 generates red and green (dual-color) luminescence from a single sample upon a single-reagent addition

Promega™ Calpain-Glo™ Assay System

Homogeneous, luminescent assay measures calpain 1 (μ) and 2 (m) activities. Adding the calpain reagent results in cleavage of the substrate and rapid development of a luminescent signal produced by a luciferase reaction. The signal is proportional to the amount of calpain activity present.

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