Reagents for Luciferase Reporter Assays

Thermo Scientific™ TurboFect™ Cell Transfection Reagent Available on GSA/VA Contract for Federal Government customers only.

Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.

Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)

Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.

BD D- Luciferin Potassium Salt Available on GSA/VA Contract for Federal Government customers only.

Synthetically made substrate which can be catalyzed by firefly luciferase

Promega Nano-Glo HiBiT Lytic Detection System, 100ml

Accurately quantify Any HiBiT Tagged Protein Expressed in Cells in an antibody free, plate based method.

Promega Nano-Glo HiBiT Lytic Detection System, 10 x 100ml

Accurately quantify Any HiBiT Tagged Protein Expressed in Cells in an antibody free, plate based method.

Promega Nano-Glo HiBiT Lytic Detection System, 10ml

Accurately quantify Any HiBiT Tagged Protein Expressed in Cells in an antibody free, plate based method.

Thermo Scientific™ Pierce™ 100X Firefly Signal Enhancer

Produce maximum bioluminescence signal intensity and sensitivity with the Pierce Firefly Luciferase Flash and Glow Assays.

Promega EnduRen In Vivo Renilla Luciferase Substrate, 3.4mg

EnduRen in vivo Renilla Luciferase Substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimize substrate degradation and autoluminescence.

BD Monolight™ Luminometer Thermal Printer Paper Available on GSA/VA Contract for Federal Government customers only.

For recording data from luminometer instrument measurements

Promega HiBiT Control Protein, 100microl

Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA.

Promega Nano-Glo In-Gel Detection System, 100ml

The Nano-Glo In-Gel Detection System directly detects the luminescence of NanoLuc fusion proteins in polyacrylamide gels after protein separation by PAGE under native or denaturing SDS conditions (native PAGE or SDS-PAGE).

EMD Millipore™ Upstate™ TCF Reporter Plasmid Kit Available on GSA/VA Contract for Federal Government customers only.

Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.

Promega™ Calpain-Glo™ Assay System

Homogeneous, luminescent assay measures calpain 1 (μ) and 2 (m) activities. Adding the calpain reagent results in cleavage of the substrate and rapid development of a luminescent signal produced by a luciferase reaction. The signal is proportional to the amount of calpain activity present.

BD D- Luciferin Sodium Salt Available on GSA/VA Contract for Federal Government customers only.

Specific for firefly luciferase in the illustrated reactions

Promega™ Chroma-Glo™ Luciferase Assay System

Homogeneous assay1, 2, 3, 4 generates red and green (dual-color) luminescence from a single sample upon a single-reagent addition

Promega™ Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System

Measures the chymotrypsin-like protease activity of the proteasome complex in cultured cells. Add the Proteasome-Glo Cell-Based Assay Reagent in an add-mix-measure format to produce proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

Promega™ VivoGlo™ Luciferin-β-Galactoside Substrate (6-O-β-galactopyranosyl luciferin)

Substrate for the commonly used reporter enzyme β-galactosidase. The substrate is cleaved by β-galactosidase to form luciferin and galactose. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light.

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