Reagents for Luciferase Reporter Assays
Thermo Scientific™ Pierce™ Luciferase Cell Lysis Buffer (2X)
Lyse adherent cells or pelleted cell suspensions for analysis of promoter expression in luciferase assays with this optimized protein extraction reagent.
Thermo Scientific™ Pierce™ 100X Firefly Signal Enhancer
Produce maximum bioluminescence signal intensity and sensitivity with the Pierce Firefly Luciferase Flash and Glow Assays.
Thermo Scientific™ TurboFect™ Cell Transfection Reagent
Deliver DNA plasmids and expression vectors into eukaryotic cells with this highly efficient, easy-to-use, non-immunogenic transfection reagent.
BD D- Luciferin Potassium Salt
Synthetically made substrate which can be catalyzed by firefly luciferase
BD Monolight™ Luminometer Thermal Printer Paper
For recording data from luminometer instrument measurements
Promega™ VivoGlo™ Luciferin-β-Galactoside Substrate (6-O-β-galactopyranosyl luciferin)
Substrate for the commonly used reporter enzyme β-galactosidase. The substrate is cleaved by β-galactosidase to form luciferin and galactose. When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light.
Promega™ Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System
Measures the chymotrypsin-like protease activity of the proteasome complex in cultured cells. Add the Proteasome-Glo Cell-Based Assay Reagent in an add-mix-measure format to produce proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.
Promega™ Chroma-Glo™ Luciferase Assay System
Homogeneous assay1, 2, 3, 4 generates red and green (dual-color) luminescence from a single sample upon a single-reagent addition
Promega™ Calpain-Glo™ Assay System
Homogeneous, luminescent assay measures calpain 1 (μ) and 2 (m) activities. Adding the calpain reagent results in cleavage of the substrate and rapid development of a luminescent signal produced by a luciferase reaction. The signal is proportional to the amount of calpain activity present.