Immunohistochemistry Reagents

BD Horizon™ BV480 Mouse Anti-Human CD38

CD38 is expressed by other cells including monocytes, macrophages, dendritic cells, NK cells, myeloid and erythroid precursors and some epithelial cells. BD Horizon™ BV480 Mouse Anti-Human CD38 antigen acts as an ectoenzyme that catalyzes the synthesis and hydrolysis of a Ca++ mobilizing agent, cyclic ADP-ribose.

BD Horizon™ BV480 Mouse Anti-Human CD4

CD4 is expressed on T-helper/inducer lymphocytes and monocytes. BD Horizon™ BV480 Mouse Anti-Human CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV). The SK3 antibody inhibits HIV binding to CD4+ cells.

BD Horizon™ BV480 Mouse Anti-Human IgD

mIgD is expressed on mature naive B cells (along with membrane IgM) and serves as a B-cell receptor for antigen (BCR). BD Horizon™ BV480 Mouse Anti-Human IgD BCR, in association with other signaling molecules including CD79a and CD79b, can transduce activating or tolerizing signals intracellularly into B lymphocytes.

BD Horizon™ BV480 Mouse Anti-Human CD3

CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. BD Horizon™ BV480 Mouse Anti-Human CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε

BD Horizon™ BV480 Mouse Anti-Human CD27

With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes. BD Horizon™ BV480 Mouse Anti-Human CD27 is present on most peripheral blood T lymphocytes and medullary thymocytes and CD27 antigen is upregulated upon activation with the release of a soluble form, 28 to 32 kDa.

BD Horizon™ BV480 Mouse Anti-Human CD45

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. BD Horizon™ BV480 Mouse Anti-Human CD45 is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.

BD Horizon™ BV480 Rat Anti-Mouse CD19

Expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Rat Anti-Mouse CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD Horizon™ BV480 Mouse Anti-Human CD138

CD138 is strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations. BD Horizon™ BV480 Mouse Anti-Human CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin, collagen types I, III, and V, tenascin, thrombospondin, and antithrombin III.

BD Horizon™ BV480 Mouse Anti-Human CD31

CD31 has wide tissue distribution and is expressed on platelets, monocytes, granulocytes, NK cells, T cell subsets, and in high amounts on endothelial cells. BD Horizon™ BV480 Mouse Anti-Human CD31 functions as a vascular endothelial cell adhesion molecule and is involved in the transendothelial migration of leucocytes in inflammatory responses.

BD Horizon™ BV480 Mouse Anti-Human CD1a

CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. BD Horizon™ BV480 Mouse Anti-Human CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation. 

BD Horizon™ BV480 Mouse Anti-Ki-67

Expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). BD Horizon™ BV480 Mouse Anti-Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear.

BD Horizon™ BV480 Mouse Anti-Human CD64

CD64 is expressed on monocytes, macrophages, dendritic cells, granulocytes activated with interferon-gamma and early myeloid lineage cells. BD Horizon™ BV480 Mouse Anti-Human CD64 associates with a signaling FcRγ homodimer to form the functional high affinity FcγRI complex and functions in both innate and adaptive immune responses.

BD Horizon™ BV480 Rat Anti-Mouse CD45

BD Horizon - BV480 Rat Anti-Mouse CD45 - CD45 is a transmembrane glycoprotein which is expressed at high levels on cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

BD Horizon™ BV480 Mouse Anti-Human CD14

CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. BD Horizon™ BV480 Mouse Anti-Human CD14-specific antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells.

BD Horizon™ BV480 Mouse Anti-Human CD127

CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. BD Horizon™ BV480 Mouse Anti-Human CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells.

BD Horizon™ BV480 Mouse Anti-Human CD4

CD4 distribution on lymphocytes is similar for both human and monkey cells. BD Horizon™ BV480 Mouse Anti-Human CD4,  with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers.

BD Horizon™ BV480 Mouse Anti-Human CD28

The CD28.2 monoclonal antibody specifically binds to CD28, a 44kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. BD Horizon™ BV480 Mouse Anti-Human CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions.

BD Horizon™ BV480 Mouse Anti-Human CD45RO

CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells. BD Horizon™ BV480 Mouse Anti-Human CD45RO and CD45RA antibodies seem to define complementary, predominantly non-overlapping, populations in resting peripheral T cells, demonstrating heterogeneity within the CD8 and CD4 subpopulations. CD45RO binds to CD22.

BD Horizon™ BV480 Mouse Anti-Human CD196 (CCR6)

CCR6 is predominantly expressed by B lymphocytes, certain subsets of effector and memory T cells and by immature dendritic cells but not by monocytes, NK cells, or granulocytyes. BD Horizon™ BV480 Mouse Anti-Human CD196 (CCR6) gene has been mapped to chromosome 6q27. CCR6 is a receptor for the CC chemokine CCL20/MIP-3alpha/LARC/Exodus and also binds with lower affinity to and mediates responses to beta-defensin2/hBD-2.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ ABC Staining Kits

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

BD Horizon™ BV480 Mouse Anti-Human CD7

CD7 is expressed on thymocytes, T cells, pre-B cells, and NK cells. BD Horizon™ BV480 Mouse Anti-Human CD7 is present in reduced density on monocytic cells and cell lines. Functional studies demonstrate that crosslinking of the CD7 can induce transmembrane calcium flux in T cells.

BD Horizon™ BV480 Rat Anti-Mouse IFN-γ

IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. BD Horizon™ BV480 Rat Anti-Mouse IFN-γ plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types.

BD Horizon™ BV480 Rat Anti-Mouse I-A/I-E

M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by I-A[b], I-A[d], I-A[q], I-E[d], I-E[k] MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. BD Horizon™ BV480 Rat Anti-Mouse I-A/I-E also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice.

BD Horizon™ BV480 Rat Anti-Mouse CD8a

CD8 is an antigen coreceptor on T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. BD Horizon™ BV480 Rat Anti-Mouse CD8a also participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]).

BD Hoechst 33342 Solution

Bioimaging certified reagent for fluorescent staining of DNA and nuclei in live or fixed cells

BD Horizon™ BV480 Mouse Anti-Human IFN-γ

IFN-γ is a potent multifunctional cytokine that is produced by several activated cell types. BD Horizon™ BV480 Mouse Anti-Human IFN- γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets.

BD Horizon™ BV480 Mouse Anti-Human IgM

IgM is an important component in the first line of defense against foreign pathogens, but may also play a role in autoimmune diseases. BD Horizon™ BV480 Mouse Anti-Human IgM monomers consist of two light and two heavy chains. Unlike the heavy chain of an IgG antibody which contains 3 constant Ig domains, the μ heavy chain of IgM contains 4 constant Ig domains.

BD Horizon™ BV480 Rat Anti-Mouse CD11b

CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). BD Horizon™ BV480 Rat Anti-Mouse CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family.

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