Immunohistochemistry Reagents

Thermo Scientific™ Pierce™ Immunostain Enhancer

Increase signal intensity 3- to 12-fold and antibody dilution 5- to 20-fold beyond normal using both chromogenic or fluorescent IHC substrates.

Thermo Scientific™ Pierce™ ABC Staining Kits

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Thermo Scientific™ Pierce™ Peroxidase Suppressor

Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.

Thermo Scientific™ Pierce™ beta-Galactosidase Precipitating Substrates

Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Bundles the Pierce Metal Enhanced DAB Substrate Kit with all components to stain, counterstain and preserve results from frozen or paraffin tissue sections.

Thermo Scientific™ Pierce™ Metal Enhanced DAB Substrate Kit

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce™ Hoechst 3342 Fluorescent Stain

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

Thermo Scientific™ Pierce™ Protein Transfection Reagent

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

BD Horizon™ BV480 Rat Anti-Mouse IgD

The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes and it does not react with other immunoglobulin isotypes. BD Horizon™ BV480 Rat Anti-Mouse IgD is expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro.

BD Horizon™ BV480 Rat Anti-Mouse CD8a

CD8 is an antigen coreceptor on T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. BD Horizon™ BV480 Rat Anti-Mouse CD8a also participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]).

BD Horizon™ BV480 Mouse Anti-Human CD45

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. BD Horizon™ BV480 Mouse Anti-Human CD45 is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.

BD Horizon™ BV480 Rat Anti-Mouse CD19

Expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Rat Anti-Mouse CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD Horizon™ BV480 Rat Anti-Human CD197 (CCR7)

CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. BD Horizon™ BV480 Rat Anti-Human CD197 (CCR7) is also detected in peripheral T and B lymphocytes, in bone marrow and cord blood CD34-positive cells and mature dendritic cells.

BD Hoechst 33342 Solution

Bioimaging certified reagent for fluorescent staining of DNA and nuclei in live or fixed cells

BD Horizon™ BV480 Mouse Anti-Human CD1a

CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. BD Horizon™ BV480 Mouse Anti-Human CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation. 

BD Horizon™ BV480 Mouse Anti-Human CD16

Expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. BD Horizon™ BV480 Mouse Anti-Human CD16 clone 3G8 also cross-reacts with a  subset of peripheral blood lymphocytes and monocytes, but not granulocytes, of baboon and both rhesus and cynomologus macaque monkeys.

BD Horizon™ BV480 Rat Anti-Mouse CD117

Widespread expression of CD117 in hematopoietic precursors is consistent with participation of c-Kit and its ligand in regulation of several hematopoietic lineages. BD Horizon™ BV480 Rat Anti-Mouse CD117 is also involved in the regulation of some events during the development of T lymphocytes. CD117 is also expressed by mast cells and by dendritic cells found in the periarteriolar lymphocytoc sheaths (T-cell areas) of splenic white pulp.

BD Horizon™ BV480 Mouse Anti-Human CD8

CD8 is expressed by majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. BD Horizon™ BV480 Mouse Anti-Human CD8 plays important roles in T cell activation and selection.

BD Horizon™ BV480 Mouse Anti-Human CD127

CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. BD Horizon™ BV480 Mouse Anti-Human CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells.

BD Horizon™ BV480 Mouse Anti-Ki-67

Expressed in the nucleus of cycling cells (G1, S, G2, M cell cycle phases). BD Horizon™ BV480 Mouse Anti-Ki-67 is a large protein having 2 alternatively spliced isoforms, an N-terminal forkhead-associated domain, a C-terminal domain that binds to heterochromatin proteins, and multiple phosphorylation sites, the functions of which are still unclear.

BD Horizon™ BV480 Mouse Anti-NHP CD45

D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. BD Horizon™ BV480 Mouse Anti-NHP CD45 antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells.  

BD Horizon™ BV480 Rat Anti-Mouse CD90.2

CD90 is a glycophosphatidylinositol-anchored membrane glycoprotein of Ig superfamily that is involved in signal transduction. BD Horizon™ BV480 Rat Anti-Mouse CD90 mediates adhesion of thymocytes to thymic stroma. The 53-2.1 antibody has been reported to block the binding of the Rat Anti-Mouse CD90.2 antibody (Clone 30-H12) to immobilized thymocyte membranes.

BD Horizon™ BV480 Mouse Anti-Human CD5

CD5 is a 67 kDa single-chain, type 1 transmembrane glycoprotein expressed on most thymocytes, the majority of peripheral T lymphocytes and a subpopulation of B cells. BD Horizon™ BV480 Mouse Anti-Human CD5+ B cells produce polyreactive antibodies (mostly IgM).

BD Horizon™ BV480 Mouse Anti-Human CD14

CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. BD Horizon™ BV480 Mouse Anti-Human CD14-specific antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells.

BD Horizon™ BV480 Mouse Anti-Human CD19

CD19 is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Mouse Anti-Human CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD Horizon™ BV480 Rat Anti-Mouse CD44

CD44 is widely expressed on hematopoietic and non-hematopoietic cells. BD Horizon™ BV480 Rat Anti-Mouse CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes.

BD Horizon™ BV480 Mouse Anti-Human CD49d

The 9F10 monoclonal antibody specifically reacts with the integrin α4 chain, that is expressed as a heterodimer with either of two β integrin subunits, β 1 (CD29) or β7. BD Horizon™ BV480 Mouse Anti-Human CD49d clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys.

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