Immunohistochemistry Reagents

Thermo Scientific™ Pierce™ Immunostain Enhancer

Increase signal intensity 3- to 12-fold and antibody dilution 5- to 20-fold beyond normal using both chromogenic or fluorescent IHC substrates.

Thermo Scientific™ Pierce™ Hoechst 3342 Fluorescent Stain

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

Thermo Scientific™ Pierce™ Protein Transfection Reagent

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

Thermo Scientific™ Pierce™ ABC Staining Kits

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Thermo Scientific™ Pierce™ beta-Galactosidase Precipitating Substrates

Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ Metal Enhanced DAB Substrate Kit

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Bundles the Pierce Metal Enhanced DAB Substrate Kit with all components to stain, counterstain and preserve results from frozen or paraffin tissue sections.

Thermo Scientific™ Pierce™ Peroxidase Suppressor

Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.

BD Horizon™ BV480 Mouse Anti-Human CD38

CD38 is expressed by other cells including monocytes, macrophages, dendritic cells, NK cells, myeloid and erythroid precursors and some epithelial cells. BD Horizon™ BV480 Mouse Anti-Human CD38 antigen acts as an ectoenzyme that catalyzes the synthesis and hydrolysis of a Ca++ mobilizing agent, cyclic ADP-ribose.

BD Horizon™ BV480 Rat Anti-Human CD197 (CCR7)

CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. BD Horizon™ BV480 Rat Anti-Human CD197 (CCR7) is also detected in peripheral T and B lymphocytes, in bone marrow and cord blood CD34-positive cells and mature dendritic cells.

BD Horizon™ BV480 Mouse Anti-NHP CD45

D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. BD Horizon™ BV480 Mouse Anti-NHP CD45 antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells.  

BD Horizon™ BV480 Mouse Anti-Human IgD

mIgD is expressed on mature naive B cells (along with membrane IgM) and serves as a B-cell receptor for antigen (BCR). BD Horizon™ BV480 Mouse Anti-Human IgD BCR, in association with other signaling molecules including CD79a and CD79b, can transduce activating or tolerizing signals intracellularly into B lymphocytes.

Phalloidin DyLight 350, Invitrogen™

Phalloidin Control, DyLight 350 conjugate, 21830, from Invitrogen™., 21830, from Invitrogen™. Species Reactivity: Many; Applications: ICC, IF, IHC.

BD Horizon™ BV480 Mouse Anti-Human CD28

The CD28.2 monoclonal antibody specifically binds to CD28, a 44kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells. BD Horizon™ BV480 Mouse Anti-Human CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions.

BD Horizon™ BV480 Mouse Anti-Human CD3

CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. BD Horizon™ BV480 Mouse Anti-Human CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε

BD Horizon™ BV480 Mouse Anti-Human IFN-γ

IFN-γ is a potent multifunctional cytokine that is produced by several activated cell types. BD Horizon™ BV480 Mouse Anti-Human IFN- γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets.

BD Horizon™ BV480 Mouse Anti-Human CD196 (CCR6)

CCR6 is predominantly expressed by B lymphocytes, certain subsets of effector and memory T cells and by immature dendritic cells but not by monocytes, NK cells, or granulocytyes. BD Horizon™ BV480 Mouse Anti-Human CD196 (CCR6) gene has been mapped to chromosome 6q27. CCR6 is a receptor for the CC chemokine CCL20/MIP-3alpha/LARC/Exodus and also binds with lower affinity to and mediates responses to beta-defensin2/hBD-2.

BD Horizon™ BV480 Mouse Anti-Human IgM

IgM is an important component in the first line of defense against foreign pathogens, but may also play a role in autoimmune diseases. BD Horizon™ BV480 Mouse Anti-Human IgM monomers consist of two light and two heavy chains. Unlike the heavy chain of an IgG antibody which contains 3 constant Ig domains, the μ heavy chain of IgM contains 4 constant Ig domains.

BD Horizon™ BV480 Mouse Anti-Human CD27

With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes. BD Horizon™ BV480 Mouse Anti-Human CD27 is present on most peripheral blood T lymphocytes and medullary thymocytes and CD27 antigen is upregulated upon activation with the release of a soluble form, 28 to 32 kDa.

BD Horizon™ BV480 Mouse Anti-Human CD62L

CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets and NK cells. BD Horizon™ BV480 Mouse Anti-Human CD62L DREG-56 antibody recognizes the same antigen as LAM-1, and specifically inhibits >90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen

sections of peripheral, but not mucosal lymphoid tissue.

BD Horizon™ BV480 Rat Anti-Mouse IgD

The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes and it does not react with other immunoglobulin isotypes. BD Horizon™ BV480 Rat Anti-Mouse IgD is expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro.

BD Horizon™ BV480 Mouse Anti-Human CD127

CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. BD Horizon™ BV480 Mouse Anti-Human CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells.

BD Horizon™ BV480 Mouse Anti-Human CD86

CD86 (B70/B7-2) is expressed primarily on monocytes, dendritic cells and activated B cells. BD Horizon™ BV480 Mouse Anti-Human CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response.

BD Horizon™ BV480 Mouse Anti-Human CD1a

CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. BD Horizon™ BV480 Mouse Anti-Human CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation. 

BD Horizon™ BV480 Mouse Anti-Human CD14

CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. BD Horizon™ BV480 Mouse Anti-Human CD14-specific antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells.

BD Horizon™ BV480 Mouse Anti-Human CD138

CD138 is strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations. BD Horizon™ BV480 Mouse Anti-Human CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin, collagen types I, III, and V, tenascin, thrombospondin, and antithrombin III.

BD Horizon™ BV480 Mouse Anti-Human CD279 (PD-1)

CD279 is an immunoregulatory receptor expressed on activated T cells, B cells, and myeloid cells. BD Horizon™ BV480 Mouse Anti-Human CD279 (PD-1) contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms.

  spinner