Immunohistochemistry Reagents

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Thermo Scientific™ Pierce™ ABC Staining Kits Available on GSA/VA Contract for Federal Government customers only.

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Thermo Scientific™ Pierce™ Immunostain Enhancer

Increase signal intensity 3- to 12-fold and antibody dilution 5- to 20-fold beyond normal using both chromogenic or fluorescent IHC substrates.

Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Bundles the Pierce Metal Enhanced DAB Substrate Kit with all components to stain, counterstain and preserve results from frozen or paraffin tissue sections.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain Available on GSA/VA Contract for Federal Government customers only.

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ Peroxidase Suppressor

Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.

Thermo Scientific™ Pierce™ Protein Transfection Reagent Available on GSA/VA Contract for Federal Government customers only.

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

Thermo Scientific™ Pierce™ Metal Enhanced DAB Substrate Kit Available on GSA/VA Contract for Federal Government customers only.

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce™ Hoechst 3342 Fluorescent Stain Available on GSA/VA Contract for Federal Government customers only.

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules Available on GSA/VA Contract for Federal Government customers only.

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ beta-Galactosidase Precipitating Substrates

Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.

BD Horizon™ BV480 Mouse Anti-Human CD31

CD31 has wide tissue distribution and is expressed on platelets, monocytes, granulocytes, NK cells, T cell subsets, and in high amounts on endothelial cells. BD Horizon™ BV480 Mouse Anti-Human CD31 functions as a vascular endothelial cell adhesion molecule and is involved in the transendothelial migration of leucocytes in inflammatory responses.

Novus Biologicals BCIP/NBT Alkaline Phosphatase Substrate

Used as substrate/chromagen in conjunction with Alkaline Phosphatase based immunostaining systems

BD Horizon™ BV480 Rat Anti-Mouse IgD

The 11-26c.2a monoclonal antibody specifically binds to mouse immunoglobulin D of all Igh-C haplotypes and it does not react with other immunoglobulin isotypes. BD Horizon™ BV480 Rat Anti-Mouse IgD is expressed on the splenic B-cell surface with high affinity, it does not induce proliferation of splenic B cells in vitro.

BD Horizon™ BV480 Rat Anti-Mouse CD44

CD44 is widely expressed on hematopoietic and non-hematopoietic cells. BD Horizon™ BV480 Rat Anti-Mouse CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes.

BD Horizon™ BV480 Mouse Anti-Human CD56

CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes. BD Horizon™ BV480 Mouse Anti-Human CD56 is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes.

BD Horizon™ BV480 Mouse Anti-Human CD27

With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes. BD Horizon™ BV480 Mouse Anti-Human CD27 is present on most peripheral blood T lymphocytes and medullary thymocytes and CD27 antigen is upregulated upon activation with the release of a soluble form, 28 to 32 kDa.

BD Horizon™ BV480 Mouse Anti-Rat CD90/Mouse CD90.1

CD90 (Thy-1) is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. BD Horizon™ BV480 Mouse Anti-Rat CD90/Mouse CD90.1 also mediates adhesion of mouse thymocytes to mouse thymic stroma. The OX-7 clone has also been reported to crossreact with rabbit and guinea pig thymus, brain, and intestine.

BD Reagent Set A Available on GSA/VA Contract for Federal Government customers only.

Use in BD OptEIA™ ELISA Sets

BD Horizon™ BV480 Mouse Anti-Human HLA-DR

HLA-DR antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. BD Horizon™ BV480 Mouse Anti-Human HLA-DR is also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4-positive T cells.

BD Horizon™ BV480 Mouse Anti-Human CD138

CD138 is strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations. BD Horizon™ BV480 Mouse Anti-Human CD138 binds to many extracellular matrix proteins through its heparan sulfate side-chains, like fibronectin, collagen types I, III, and V, tenascin, thrombospondin, and antithrombin III.

BD Horizon™ BV480 Rat Anti-Mouse IFN-γ

IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. BD Horizon™ BV480 Rat Anti-Mouse IFN-γ plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types.

BD Horizon™ BV480 Mouse Anti-Human CD8

CD8 is expressed by majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells. BD Horizon™ BV480 Mouse Anti-Human CD8 plays important roles in T cell activation and selection.

BD Horizon™ BV480 Mouse Anti-Human CD45RA

CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. BD Horizon™ BV480 Mouse Anti-Human CD45RA antigen is expressed by naive and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.

BD Horizon™ BV480 Mouse Anti-Human CD14

CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. BD Horizon™ BV480 Mouse Anti-Human CD14-specific antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells.

BD Horizon™ BV480 Mouse Anti-Human CD49d

The 9F10 monoclonal antibody specifically reacts with the integrin α4 chain, that is expressed as a heterodimer with either of two β integrin subunits, β 1 (CD29) or β7. BD Horizon™ BV480 Mouse Anti-Human CD49d clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys.

BD Horizon™ BV480 Mouse Anti-NHP CD45

D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. BD Horizon™ BV480 Mouse Anti-NHP CD45 antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells.  

BD Horizon™ BV480 Rat Anti-Mouse CD45

BD Horizon - BV480 Rat Anti-Mouse CD45 - CD45 is a transmembrane glycoprotein which is expressed at high levels on cell-surface, and its presence distinguishes leukocytes from non-hematopoietic cells. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction and the CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific.

BD Horizon™ BV480 Rat Anti-Mouse CD117

Widespread expression of CD117 in hematopoietic precursors is consistent with participation of c-Kit and its ligand in regulation of several hematopoietic lineages. BD Horizon™ BV480 Rat Anti-Mouse CD117 is also involved in the regulation of some events during the development of T lymphocytes. CD117 is also expressed by mast cells and by dendritic cells found in the periarteriolar lymphocytoc sheaths (T-cell areas) of splenic white pulp.

BD Horizon™ BV480 Rat Anti-Mouse I-A/I-E

M5/114.15.2 monoclonal antibody recognizes a polymorphic determinant shared by I-A[b], I-A[d], I-A[q], I-E[d], I-E[k] MHC class II alloantigens that can be expressed by B cells, dendritic cells, monocytes, macrophages and activated T cells. BD Horizon™ BV480 Rat Anti-Mouse I-A/I-E also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice.

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