Immunohistochemistry Reagents

Thermo Scientific™ Pierce™ LDH Cytotoxicity Assay Kit Available on GSA/VA Contract for Federal Government customers only.

Quantitatively measure lactose dehydrogenase released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay.

Thermo Scientific™ Pierce™ Methanol-free Formaldehyde Ampules Available on GSA/VA Contract for Federal Government customers only.

Vials of high-quality, methanol-free formaldehyde, a general IHC fixative and reversible amine-crosslinking agent for protein and nucleic acid samples.

Thermo Scientific™ Pierce™ DAPI Nuclear Counterstain Available on GSA/VA Contract for Federal Government customers only.

Highly pure diamidino-2-phenylindole (DAPI) dye for fluorescent staining of DNA content and nuclei in fixed-cell imaging techniques.

Thermo Scientific™ Pierce™ Hoechst 3342 Fluorescent Stain Available on GSA/VA Contract for Federal Government customers only.

High-quality Hoechst dye solution for fluorescent staining of DNA and nuclei in fixed- or live-cell imaging techniques.

Invitrogen™ eBioscience™ Fixation/Permeabilization Concentrate Non-distribution product as customer accommodation.

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

Thermo Scientific™ Pierce™ Metal Enhanced DAB Substrate Kit Available on GSA/VA Contract for Federal Government customers only.

Special formulation of diaminobenzidine peroxidase substrate that uses cobalt and nickel chloride to yield intense color for IHC staining.

Thermo Scientific™ Pierce™ Protein Transfection Reagent Available on GSA/VA Contract for Federal Government customers only.

Form complexes with proteins, peptides, antibodies and other biologically active molecules for direct intracellular delivery using this cationic lipid mixture.

Thermo Scientific™ Pierce™ ABC Staining Kits Available on GSA/VA Contract for Federal Government customers only.

Detect biotinylated secondary antibodies by IHC using the highly sensitive avidin-biotin complex (ABC) technique.

Thermo Scientific™ Pierce™ Immunostain Enhancer

Increase signal intensity 3- to 12-fold and antibody dilution 5- to 20-fold beyond normal using both chromogenic or fluorescent IHC substrates.

Novex™ Endogenous Biotin-Blocking Kit Available on GSA/VA Contract for Federal Government customers only.

Endogenous Biotin-Blocking Kit

Invitrogen™ Click-iT™ TUNEL Colorimetric IHC Detection Kit

Click-iT TUNEL Colorimetric IHC Detection Kit

Meridian Bioscience™ Latex Agglutination System Available on GSA/VA Contract for Federal Government customers only.

Qualitative and semi-quantitative latex test for detection of Cryptococcus neoformans

Molecular Probes™ Human Neural Stem Cell Immunocytochemistry Kit Available on GSA/VA Contract for Federal Government customers only.

Human Neural Stem Cell Immunocytochemistry Kit

Thermo Scientific™ Pierce™ Peroxidase IHC Detection Kit

Bundles the Pierce Metal Enhanced DAB Substrate Kit with all components to stain, counterstain and preserve results from frozen or paraffin tissue sections.

BD BV480 Mouse Anti-Human CD19

CD19 is expressed throughout B-lymphocyte development from the pro-B cell through the mature B-cell stages. BD Horizon™ BV480 Mouse Anti-Human CD19 participates in B-lymphocyte development, B-cell activation, maturation of memory B cells and regulation of tolerance. CD19 has also been detected on peritoneal mast cells, co-localized with CD21/CD35, and it is proposed to play a role in complement-mediated mast-cell activation.

BD BV480 Rat Anti-Mouse CD8a

CD8 is an antigen coreceptor on T-cell surface which interacts with MHC class I molecules on antigen-presenting cells or epithelial cells. BD Horizon™ BV480 Rat Anti-Mouse CD8a also participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck (p56 [lck]).

BD BV480 Mouse Anti-Human CD45

CD45 is encoded by the PTPRC (Protein tyrosine phosphatase receptor type C) gene. BD Horizon™ BV480 Mouse Anti-Human CD45 is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues.

EMD Millipore™ Black Gold II Myelin Staining Kit Available on GSA/VA Contract for Federal Government customers only.

Stains specifically for myelin within the central nervous system, provides high resolution, high contrast, a short histochemical processing time, and high reproducibility.

BD BV480 Mouse Anti-Human CD25

CD25 is expressed on regulatory T cells, activated lymphocytes (T and B), and monocytes. BD Horizon™ BV480 Mouse Anti-Human CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation.

BD Reagent Set A Available on GSA/VA Contract for Federal Government customers only.

Use in BD OptEIA™ ELISA Sets

Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution, High pH, 10x Non-distribution product as customer accommodation.

Designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. Use in combination with heat has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue.

BD BV480 Mouse Anti-Human CD27

With its ligand CD70, CD27 acts in a co-stimulatory fashion on T lymphocytes. BD Horizon™ BV480 Mouse Anti-Human CD27 is present on most peripheral blood T lymphocytes and medullary thymocytes and CD27 antigen is upregulated upon activation with the release of a soluble form, 28 to 32 kDa.

BD BV480 Mouse Anti-Human CD49d

The 9F10 monoclonal antibody specifically reacts with the integrin α4 chain, that is expressed as a heterodimer with either of two β integrin subunits, β 1 (CD29) or β7. BD Horizon™ BV480 Mouse Anti-Human CD49d clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys.

Thermo Scientific™ Pierce™ Peroxidase Suppressor

Stable, easy-to-use endogenous peroxidase inhibitor to reduce background signal from cells and tissues in HRP-based IHC staining.

EMD Millipore™ Black Gold II Stain Available on GSA/VA Contract for Federal Government customers only.

Stains specifically for myelin within the central nervous system, provides high resolution, high contrast, a short histochemical processing time, and high reproducibility.

Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution, Low pH, 10x Non-distribution product as customer accommodation.

Designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. Used in combination with heat has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue.

BD BV480 Mouse Anti-Human CD3

CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. BD Horizon™ BV480 Mouse Anti-Human CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε

Molecular Probes™ Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit Available on GSA/VA Contract for Federal Government customers only.

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit

Invitrogen™ eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 660 Non-distribution product as customer accommodation.

Contains reagents and buffers to identify and examine proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and formalin-fixed paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

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