Miscellaneous Molecular Reagents

Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling Available on GSA/VA Contract for Federal Government customers only.

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Thermo Scientific™ Pierce™ MS Grade Trypsin Protease Available on GSA/VA Contract for Federal Government customers only.

Ensure maximum activity and stability in proteomic applications with this chemically modified, highly purified porcine trypsin.

Thermo Scientific™ Pierce™ Amine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

For multiplex quantitative protein expression analysis by mass spectrometry

Thermo Scientific™ Pierce™ BS3-d0 and BS3-d4 Crosslinker Mass Pair

Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.

Thermo Scientific™ Pierce™ D-Luciferin Available on GSA/VA Contract for Federal Government customers only.

Firefly luciferase substrate for in vitro and in vivo applications

Thermo Scientific™ Pierce™ Amine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

Study protein expression by quantitative mass spectrometry using amine-labeling compounds of equal total mass (isobaric) but different reporter ions.

Thermo Scientific™ Pierce™ LTQ Velos ESI Positive Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ Velos, LTQ Orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

Thermo Scientific™ IPTG Available on GSA/VA Contract for Federal Government customers only.

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

Thermo Scientific™ Pierce™ HeLa Protein Digest Standard Available on GSA/VA Contract for Federal Government customers only.

Use this standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Thermo Scientific™ Pierce™ Hydrochloric Acid Solution Available on GSA/VA Contract for Federal Government customers only.

Constant-boiling 6N HCl solution, supplied in 1mL ampules, used to acid-hydrolyze proteins (protein hydrolysis) for HPLC amino acid composition analysis.

Thermo Scientific™ SILAC Protein Quantitation Kit (LysC) - DMEM

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ DMEM Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit.

Thermo Scientific™ Pierce™ DTNB (Ellman's Reagent)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Pierce™ Vacuum Hydrolysis Tubes

High-quality valved glass tubes designed for heated, vacuum, acid-hydrolysis of proteins and peptides for amino acid analysis by HPLC chromatography.

Thermo Scientific™ TransformAid Bacterial Transformation Kit

Kit for quick and convenient preparation of competent E.coli cells for guaranted transformation efficiencies of more than 107 transformants per µg of plasmid DNA.

Thermo Scientific™ High-Select™ Phosphopeptide Enrichment Kit

Enriches phosphopeptides from complex samples.

Thermo Scientific™ Pierce™ Cysteine-Reactive Tandem Mass Tag Reagents Available on GSA/VA Contract for Federal Government customers only.

Perform measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.

Thermo Scientific™ Pierce™ C-18 Spin Columns Available on GSA/VA Contract for Federal Government customers only.

Desalt, clean up and prep 25 to 150 microliter peptide samples for mass spectrometry with these ready-to-use microcentrifuge columns of C18 reverse-phase resin.

Thermo Scientific™ Pierce™ TNBSA (Trinitrobenzene Sulfonic Acid)

Label primary amines (peptides or amino acids) for colorimetric detection (Amax 335nm) using this 5% solution of trinitrobenzene sulfonic acid in methanol.

Thermo Scientific™ RPMI 1640 Media for SILAC

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ Fluoraldehyde (OPA) Reagent Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use solution of o-phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent to measure peptides or amino acids (Ex 340nm, Em 455nm).

Invitrogen™ Moleular Probes™ Click-iT™ Plus OPP Alexa Fluor™ 647 Protein Synthesis Assay Kit Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Provides non-radioactive method for detection of protein synthesis using fluorescence microscopy or high-content imaging

Thermo Scientific™ SILAC Protein Quantitation Kit (LysC) - RPMI 1640

Analyze protein expression by mass spectrometry using these media or additives for stable isotope labeling with amino acids in cell culture (SILAC).

Thermo Scientific™ Pierce™ ESI Negative Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ mass spectrometer instruments using a mixture of highly purified ionizable molecules designed for negative mode calibration.

Thermo Scientific™ Pierce™ FDAA (Marfey's Reagent)

Label and measure optical isomers of amino acids by reverse-phase chromatography (HPLC) with this amine-reactive, colored (Amax 340nm) compound.

Thermo Scientific™ Immobilized Anti-TMT™ Antibody Resin

Thermo Scientific™ Pierce™ Anti-TMT Antibody Resin enables selective enrichment of peptides in complex samples that have been labeled with Tandem Mass Tag™ Reagents.

Thermo Scientific™ TMT™ Elution Buffer

Thermo Scientific™ TMT™ Elution Buffer is for use with Thermo Scientific™ Immobilized Anti-TMT™ Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.

Thermo Scientific™ Pierce™ LTQ ESI Positive Ion Calibration Solution Available on GSA/VA Contract for Federal Government customers only.

Calibrate LTQ, LTQ Orbitrap, LXQ and LCQ FLEET mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration.

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