Western Blotting Reagents, Membranes, Supplies

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

EMD Millipore Immobilon™-P PVDF Transfer Membranes Available on GSA/VA Contract for Federal Government customers only.

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ Pierce™ SuperSignal West Dura Fast Western Blot Kits

For femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

EMD Millipore Immobilon™ PVDF Transfer Membranes: PSQ Available on GSA/VA Contract for Federal Government customers only.

For Western blots and protein sequencing

EMD Millipore BlØk™ Noise Canceling Reagents Available on GSA/VA Contract for Federal Government customers only.

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Thermo Scientific™ Pierce™ Fast Western Blot Kits, ECL

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

Thermo Scientific™ Pierce™ SuperSignal West Femto Fast Western Blot Kit

For mid-to-low femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Avidin Biotin Polyclonal Antibody, Invitrogen™

Avidin Antibody, Biotin conjugate, 31850, from Invitrogen™. Species Reactivity: Ck; Applications: ELISA, IHC, WB.

EMD Millipore Immobilon™-P Blotting Sandwiches Available on GSA/VA Contract for Federal Government customers only.

Convenient, time-saving option for preparing multiple blots

EMD Millipore Immobilon™-FL PVDF Transfer Membranes Available on GSA/VA Contract for Federal Government customers only.

Designed specifically for Western blots and protein sequencing. EMD Millipore Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ SuperSignal™ West Pico Chemiluminescent Substrate

Enhanced chemiluminescent (ECL) HRP substrate that provides low-picogram-level detection by Western blot analysis.

Thermo Scientific™ Owl™ Blotting Paper

Absorb adequate amounts of buffer for semi-dry electroblotting without the loss of mechanical stability with this high-quality blotting paper.

Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce™ SuperSignal West Pico Fast Western Blot Kit

For picogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ Pierce™ S-Nitrosylation Western Blot Kit

Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with a complete, easy-to-use kit.

Thermo Scientific™ HisProbe™-HRP Reagent and Kit

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Thermo Scientific™ Pierce™ Far-Western Blot Kit for Biotinylated Proteins

Detect proteins in gels or on membranes via interaction with biotin-labeled bait proteins as primary probes.

Thermo Scientific™ SuperSignal™ Western Blotting Kits

Combines SuperSignal West Pico substrate with HRP-conjugated rabbit or mouse secondary antibodies for a convenient, easy-to-use Western blot detection kit.

Thermo Scientific™ Clean-Blot IP Detection Reagents and Kit

Avoid interference from denatured IP antibody fragments in Western blots of immunoprecipitated proteins with these HRP and AP conjugates.

Thermo Scientific™ Pierce™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

EMD Millipore Luminata™ Western HRP Chemiluminescence Substrates Available on GSA/VA Contract for Federal Government customers only.

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Thermo Scientific™ Pierce™ Chloronaphthol (CN) Substrates

Powders, tablets and ready-made 4-chloro-1-naphthol solutions for chromogenic HRP detection in Western blot and tissue staining methods.

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

EMD Millipore Immobilon™ Blotting Filter Paper Available on GSA/VA Contract for Federal Government customers only.

Chromatography-grade blotting filter paper precut to the most popular Western blotting sizes

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