Western Blotting Reagents, Membranes, Supplies
Western Blotting Detection Reagents
Find what you need to support your Western blotting workflow, including nitrocellulose membranes, detection reagents, and more.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. After electrophoresis, the proteins in the gel are transferred to a membrane. The memabrane is then covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
The keys steps of western blotting include:
- Separate proteins by molecular weight using gel electrophoreses
- Protein gels, ladders, and stains
- Western blotting reagents and buffers
- Chamber systems
- Transfer the protein from the gel to a solid-support membrane
- Wet, semi-dry, and dry transfer systems
- Western blotting membranes
- Detect proteins visually using antibodies and substrates
- Antibodies and detection reagents
- Western blot buffers
- Imaging systems
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