Western Blotting Reagents, Membranes, Supplies

EMD Millipore™ Immobilon™-P PVDF Transfer Membranes Available on GSA/VA Contract for Federal Government customers only.

Optimized for binding proteins transferred from a variety of gel matrices. EMD Millipore Immobilon™-P PVDF Transfer Membranes have superior handling, staining, and resistance than nitrocellulose. For use with Western blots and protein sequencing. Available in sheets or rolls.

Thermo Scientific™ SuperSignal™ West Femto Chemiluminescent Substrate Available on GSA/VA Contract for Federal Government customers only.

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

EMD Millipore™ Immobilon™ FL PVDF Transfer Membranes Available on GSA/VA Contract for Federal Government customers only.

Designed specifically for Western blots and protein sequencing. EMD Millipore Immobilon™-FL PVDF Transfer Membranes are plain surfaced with 0.45μm pores and have lower background fluorescence than other membranes. Available in 10cm and 20cm squares and a 3.75m roll.

Invitrogen™ Invitrolon Novex Invitrolon PVDF/Filter Paper Sandwich Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Pre-cut and pre-assembled and ready for protein blotting/transfer

Thermo Scientific™ Clean-Blot IP Detection Reagents and Kit

Avoid interference from denatured IP antibody fragments in Western blots of immunoprecipitated proteins with these HRP and AP conjugates.

Invitrogen™ Novex™ XCell™ II Blot Vertical Module Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Easy transfer of proteins or nucleic acids from mini-gels to membranes

Thermo Scientific™ SuperSignal™ West Dura Chemiluminescent Substrate Available on GSA/VA Contract for Federal Government customers only.

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Thermo Scientific™ PVDF Transfer Membrane, 0.2 µm, 26.5 cm x 3.75 m Available on GSA/VA Contract for Federal Government customers only.

Thermo Scientific Pierce PVDF Transfer Membranes are made of high-quality polyvinylidene difluoride and provide high binding capacity for proteins and nucleic acids for Western, Southern, and Northern blotting methods. This PVDF membrane has a 0.2-micron pore size and is ideal for colorimetric and chemiluminescent detection.

Thermo Scientific™ Pierce™ TMB Substrate Solution Available on GSA/VA Contract for Federal Government customers only.

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

Thermo Scientific™ HisProbe™-HRP Reagent and Kit Available on GSA/VA Contract for Federal Government customers only.

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

EMD Millipore™ Immobilon™ PVDF Transfer Membranes: PSQ Available on GSA/VA Contract for Federal Government customers only.

For Western blots and protein sequencing

Invitrogen™ Novex™ Nitrocellulose Pre-Cut Blotting Membranes Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Ideal for blotting proteins and nucleic acids

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate Available on GSA/VA Contract for Federal Government customers only.

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Invitrogen™ Novex™ PVDF Pre-cut Blotting Membranes Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Pre-cut and pre-assembled—ready for protein blotting/transfer

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls Available on GSA/VA Contract for Federal Government customers only.

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Pierce™ Fast Western Blot Kits, ECL

Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent Available on GSA/VA Contract for Federal Government customers only.

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ Pierce™ SuperSignal West Pico Fast Western Blot Kit

For picogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ Pierce™ S-Nitrosylation Western Blot Kit

Perform sensitive detection of protein S-nitrosocysteine post-translational modifications with a complete, easy-to-use kit.

Invitrogen™ iBind™ Flex Western Device Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Allows better antigen-antibody interaction and solutions flow between the iBind™ Card and membrane for maximum interaction between protein and antibody

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate Available on GSA/VA Contract for Federal Government customers only.

Recommended HRP substrate for fluorescence imagers

Invitrogen™ iBind Flex Card Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Card for use with iBind™ Flex Western Blot Device

GE Healthcare Amersham™ Hybond™ -N+ Membranes Available on GSA/VA Contract for Federal Government customers only.

Use for the highest sensitivity in DNA and RNA blotting. GE Healthcare Life Sciences™ Amersham™ Hybond™ -N+ Membrane is a positively charged nylon membranes for use with radioactive and nonradioactive chemiluminescence and chemifluorescence detection.

Thermo Scientific™ Pierce™ NBT/BCIP Substrate Solutions Available on GSA/VA Contract for Federal Government customers only.

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce™ SuperSignal West Dura Fast Western Blot Kits

For femtogram-level detection and Western blot development less than 1 hour after gel transfer.

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls Available on GSA/VA Contract for Federal Government customers only.

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

EMD Millipore™ Luminata™ Western HRP Chemiluminescence Substrates Available on GSA/VA Contract for Federal Government customers only.

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Invitrogen™ Novex™ Invitrolon™ Novex Invitrolon PVDF/Filter Paper Sandwich Available on GSA/VA Contract for Federal Government customers only. Non-distribution product as customer accommodation.

Make blotting of E-PAGE™ Gels easier and more efficient

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane Available on GSA/VA Contract for Federal Government customers only.

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer Available on GSA/VA Contract for Federal Government customers only.

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter

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