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Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate
Recommended HRP substrate for fluorescence imagers
| Content And Storage | 2°C to 8°C |
|---|---|
| Kit Contents | Detection reagent 1 and 2 |
| Product Type | ECL Western Blot Substrate |
Invitrogen™ iBlot™ 3 Western Blot Transfer Device
The iBlot 3 Western Blot Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably in as few as three minutes and without the need for liquid buffers.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Type | Transfer Device |
|---|---|
| For Use With (Equipment) | iBlot 3 Transfer Stacks |
| Running Time | ∼3 to 10 min. |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Throughput | 2 Midi or 4 Mini Gels |
| For Use With (Application) | Western Blot |
| Mode of Transfer | Dry |
| Gel Compatibility | Mini- or Midi-sized: Bis-Tris Gels, Tricine Gels, Tris-Acetate Gels, and Tris-Glycine Gels |
| Product Line | iBlot 3 |
Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate
Optimized reagents that shorten the time to perform a typical Western blot from 4 hours to approximately 55 minutes.
| Substrate | Horseradish Peroxidase |
|---|---|
| Reactivity | Mouse, Rabbit |
| Conjugate | HRP |
| Form | Liquid |
| Product Type | Western Blot Kit |
| Membrane Compatibility | Nitrocellulose, PVDF |
| For Use With (Application) | Western Blot |
| Product Line | Pierce |
| Detection Method | Chemiluminescence |
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.
| Content And Storage | 2°C to 8°C |
|---|---|
| Sensitivity | ∼10 pg |
| Product Type | ECL Western Blot Detection Reagent |
| For Use With (Application) | Western Blot |
Invitrogen™ WesternDot™ 625 Western Blot Kits
The WesternDot 625 Western Blot kits combine the unique properties of Qdot 625 nanocrystals with the high-affinity streptavidin-biotin interaction to allow simple, highly sensitive detection of proteins on western blots.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Room Temperature |
|---|---|
| For Use With (Equipment) | iBlot, Photographic Imager |
| Sample Type | Protein, Cell Extracts |
| Label or Dye | Qdot 625 |
| Color | Red |
| Quantity | 1 kit |
| Shelf Life | 6 Months |
| Label Type | Qdot Nanocrystals |
| Detection Location | In-Blot Detection |
| Detection Method | Fluorescence |
Thermo Scientific™ Western Blot Roller
A simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.
Invitrogen™ Bandmate™ Automated Western Blot Processor
The Bandmate Automated Western Blot Processor is a benchtop instrument that automatically performs the hands-on steps of western blot processing, including blocking, washing, and antibody incubations.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Pierce™ ECL Western Blotting Substrate
Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
| Substrate | Horseradish Peroxidase |
|---|---|
| Content And Storage | Upon receipt store at 2-8°C. Product is shipped at ambient temperature. |
| Form | Liquid |
| Product Type | Substrate |
| Signal Duration | 1 to 2 hr. |
| Product Line | Pierce |
| Detection Method | Chemiluminescence |
Thermo Scientific™ SuperSignal™ Western Blot Enhancer
Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.
| Content And Storage | Upon receipt store solutions at 4°C Products are shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Type | Western Blot Enhancer |
| Membrane Compatibility | Nitrocellulose, PVDF |
| For Use With (Application) | Western Blot |
| Product Line | SuperSignal |
| Detection Method | Chemiluminescence, Fluorescence, Colorimetric |
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
| Content And Storage | Upon receipt store at 4°C. Product shipped on ice. |
|---|---|
| Sensitivity | Low picogram to mid-femtogram |
| Form | Liquid |
| Product Type | Substrate |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | Pierce |
| Detection Method | Chemiluminescence, Fluorescence |
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific™ Western Blotting Filter Paper, 0.83 mm thick, 7 x 8.4 cm
Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.
Thermo Scientific™ Restore™ Western Blot Stripping Buffer
Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
| Content And Storage | Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer
Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.
| Content And Storage | Upon receipt store at room temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |
Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
| Content And Storage | Upon receipt store at room temperature. Product shipped at ambient temperature. |
|---|---|
| Form | Liquid |
| Product Line | Restore |