Search results for "iba+immobilization+strepMab-immo"
IBA LifeSciences StrepMAB-Immo conjugate DY-649; 50 µg
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649). The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, StrepMAB-Classic or Strep-Tactin are recommended.
IBA LifeSciences StrepMAB-Immo monoclonal antibody; 100 µg
StrepMAB-Immo is a murine, high-affinity monoclonal antibody, which is especially suited for stable, mild, and oriented capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases (e.g. Biacore CM5 sensor chips) or for detection of those proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing the Strep-tagII with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). Please note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin is recommended.
IBA LifeSciences StrepMAB-Immo conjugate DY-488; 50 µg
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649). The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, StrepMAB-Classic or Strep-Tactin are recommended.
IBA LifeSciences StrepMAB-Classic monoclonal antibody; 100 µg
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available unconjugated, coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions. The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences StrepMAB-Classic monoclonal antibody with HRP conjugate; 75 µl
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences Strep-tagII peptide; 1.8 mg
Synthetic Strep-tagII peptide recognized by Strep-Tactin, Strep-TactinXT as well as the monoclonal antibodies, StrepMAB-Classic and StrepMAB-Immo. It can be used for competitive elution of Strep-tagII fusion proteins or as control protein in dot blot assays.
IBA LifeSciences StrepMAB-Classic conjugate DY-649; 50 µg
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences StrepMAB-Classic conjugate DY-488; 50 µg
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences Streptavidin; 100 mg
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or conjugated detection reagents. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Streptavidin; 1 g
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or conjugated detection reagents. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Streptavidin; 10 mg
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or conjugated detection reagents. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Streptavidin lyophilized
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or detection reagents conjugated with, e.g., fluorescent dyes. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Strep-TactinXT 4Flow HC
Strep-TactinXT 4Flow high capacity columns are applicable to the purification of Strep-tagII or Twin-Strep-tag fusion proteins via gravity flow. It is recommended for proteins of all classes, especially low abundant, large, and highly expressed proteins, due to its pH compatibility, target purity (> 95%), and high binding affinity. Multimeric protein complexes might bind too strongly to our Strep-TactinXT 4Flow high capacity resins, preventing efficient elution. Strep-Tactin resins are recommended for these proteins.With Strep-TactinXT 4Flow high capacity, denaturing agents such as urea can be used. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a higher yield of purified protein.Purify large samples faster with IBA's WET FRED.
ACROBiosystems Mouse CD3 epsilon&CD3 gamma Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (MALS verified)
Recombinant Protein;100UG;Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (CDG-M52W2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P22646-1 (CD3E) & P11942-1 (CD3G)).;Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 41.5 kDa (CD3E) and 41.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. As a result of glycosylation, the protein migrates as 48-55 kDa under reducing (R) condition, and 100 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Mouse CD3E&CD3G Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-M52W2) at 1 μg/mL (100 μL/well) can bind Biotinylated CD3e Monoclonal Antibody (Clone : 145-2C11) with a linear range of 0.2-6 ng/mL (QC tested).;Immo