Search results for "iba immobilization strepMab-immo"
IBA LifeSciences StrepMAB-Immo conjugate DY-649; 50 µg
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649). The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, StrepMAB-Classic or Strep-Tactin are recommended.
IBA LifeSciences StrepMAB-Immo monoclonal antibody; 100 µg
StrepMAB-Immo is a murine, high-affinity monoclonal antibody, which is especially suited for stable, mild, and oriented capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases (e.g. Biacore CM5 sensor chips) or for detection of those proteins in applications like ELISA, FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker (recombinant protein-SA-WSHPQFEK or SA-WSHPQFEK-recombinant protein). Proteins containing the Strep-tagII with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). Please note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, the use of StrepMAB-Classic or Strep-Tactin is recommended.
IBA LifeSciences StrepMAB-Immo conjugate DY-488; 50 µg
StrepMAB-Immo fluorescenct conjugates are murine, high-affinity monoclonal antibodies, which are especially suited for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like FACS, immunohistochemistry, and immunocytochemistry. The nearly irreversible binding is validated for fusion proteins carrying a C- or N-terminal Strep-tagII providing an N-terminal extension by a SerAla linker. However, the Twin-Strep-tag always contains the SerAla linker (WSHPQFEK-GGGS-GGGS-GGSA-WSHPQFEK). StrepMAB-Immo is available as conjugate with different fluorescent dyes (DY-488, DY-549, DY-649). The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For detection of Strep-tagII or Twin-Strep-tag fusion proteins in western blots, StrepMAB-Classic or Strep-Tactin are recommended.
IBA LifeSciences StrepMAB-Classic monoclonal antibody; 100 µg
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available unconjugated, coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions. The absorption/emission maxima of the fluorescent dyes are: • DY-488: 493/518 nm • DY-549: 550-560/575 nm • DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences StrepMAB-Classic monoclonal antibody with HRP conjugate; 75 µl
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences Twin-Strep-tag Capture Kit for SPR analysis; Classic size
Twin-Strep-tag Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-TactinXT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-TactinXT:Twin-Strep-tag interaction, which operates with pM affinity. The key benefits are:• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure• High affinity enabling measurements over a long period• Chip can be regenerated easily by an established procedure• Ligands can be captured directly from complex samples due to the high affinity and specificityThe use of unconjugated Strep-TactinXT requires a license that is provided with the purchase of the kit as limited use license.
IBA LifeSciences StrepMAB-Classic conjugate DY-649; 50 µg
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences StrepMAB-Classic conjugate DY-488; 50 µg
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tagII and Twin-Strep-tag fusion proteins in applications like western blots, ELISA, FACS, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.The absorption/emission maxima of the fluorescent dyes are:• DY-488: 493/518 nm• DY-549: 550-560/575 nm• DY-649: 655/676 nmPlease note: For capturing of Strep-tagII and Twin-Strep-tag fusion proteins on solid phases, the use of StrepMAB-Immo or Strep-TactinXT is recommended due to the binding affinity in the pM range.
IBA LifeSciences Strep-tagII peptide; 1.8 mg
Synthetic Strep-tagII peptide recognized by Strep-Tactin, Strep-TactinXT as well as the monoclonal antibodies, StrepMAB-Classic and StrepMAB-Immo. It can be used for competitive elution of Strep-tagII fusion proteins or as control protein in dot blot assays.
IBA LifeSciences Streptavidin; 100 mg
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or conjugated detection reagents. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Streptavidin; 1 g
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or conjugated detection reagents. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Streptavidin; 10 mg
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. The streptavidin:biotin system is widely used for immobilization and detection of biotinylated molecules. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or conjugated detection reagents. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Streptavidin lyophilized
Streptavidin is a tetrameric protein composed of identic subunits. Each subunit specifically binds one biotin molecule with fM affinity, which makes it one of the strongest non-covalent interactions known. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties regarding homogeneity, solubility, resistance towards proteolytic degradation, and accessibility of the biotin binding pocket as compared to native streptavidin. IBA Lifesciences provides bulk amounts of lyophilized streptavidin in reliable and high quality at competitive prices to generate streptavidin-coated surfaces (microplates, SPR chips, beads) or detection reagents conjugated with, e.g., fluorescent dyes. Please note that streptavidin is not applicable for detection, immobilization, and purification of Strep-tagII and Twin-Strep-tag fusion proteins, since the binding affinity for both tags is too low.
IBA LifeSciences Strep-TactinXT 4Flow HC
Strep-TactinXT 4Flow high capacity columns are applicable to the purification of Strep-tagII or Twin-Strep-tag fusion proteins via gravity flow. It is recommended for proteins of all classes, especially low abundant, large, and highly expressed proteins, due to its pH compatibility, target purity (> 95%), and high binding affinity. Multimeric protein complexes might bind too strongly to our Strep-TactinXT 4Flow high capacity resins, preventing efficient elution. Strep-Tactin resins are recommended for these proteins.With Strep-TactinXT 4Flow high capacity, denaturing agents such as urea can be used. In comparison to Strep-TactinXT 4Flow, Strep-TactinXT 4Flow high capacity has a higher density of immobilized Strep-TactinXT, which leads to a higher yield of purified protein.Purify large samples faster with IBA's WET FRED.
IBA LifeSciences GFP-Strep-tag control protein Strep-tagII; 500 µg
GFP-Strep-tagII is a control protein consisting of the green fluorescent protein (GFP) C-terminally tagged with the Strep-tagII. It is suitable for test purifications, detections, and immobilizations with Strep-Tactin or Strep-TactinXT.
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