Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA Available on GSA/VA Contract for Federal Government customers only.

Rapidly express full-length, functional proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction using these cell-free kits.

$232.50 - $897.00


Products 2
Catalog Number Mfr. No. Description Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Description Quantity Price Quantity & Availability  
PI88881
View Documents
Thermo Scientific™
88881
Pierce 1-Step Human Coupled IVT Kit - DNA 8 rxn kit
Each for $232.50
Add to cart
 
PI88882 Available on GSA/VA Contract for Federal Government customers only.
View Documents
Thermo Scientific™
88882
Pierce 1-Step Human Coupled IVT Kit - DNA 40 rxn kit
Each for $897.00
Add to cart
 
Description

Description

The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction.

The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100μg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.

Highlights:

Functional – uses the human translational machinery to express active proteins

  • Convenient – perform transcription and translation in a single step
  • High performance – greater yields compared to rabbit reticulocyte in vitro translation
  • Reliable – express proteins that fail in rabbit reticulocyte systems

Better Than Traditional Methods:

  • HeLa cell-free extracts are capable of expressing proteins with post-translational modifications
  • Accurate translation delivers full-length protein compatible with downstream applications
  • Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements

Includes:

Complete kits contain HeLa cell lysate, accessory proteins, reaction mix, nuclease-free water, expression vector and a GFP-positive control vector

Requires:

30°C incubator or water bath

Recommended for:

Express proteins to measure enzyme activity; Perform mutational analysis; Express protein for use in gel mobility shift assays; Perform co-immunoprecipitation; Express cytotoxic proteins; Perform unnatural amino acid labeling

Safety and Handling

Safety and Handling

SDS
Product Certifications
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit