Abnova™ 1p36/1q25 CISH Probe

Description

1p36/1q25 CISH Probe is designed for the qualitative detection of human chromosome 1p36.31 and 1q25.3 specific sequences in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (1q25 region), and dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (1p36 region).
Normal situation: In interphases of normal cells or cells without aberrations of chromosome 1, two red (1p36) signals and two green (1q25) signals appear.
Aberrant situation: In a cell with deletions affecting the 1p36 locus, one or no copy of the red signal will be observed.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P