3,3',5,5'-Tetramethylbenzidine Solution, High Sensitivity, PROMO, Thermo Scientific Chemicals

Description

3,3’,5,5’-Tetramethylbenzidine is a blue chromogenic substance used in staining techniques such as immunohistochemistry.

General Description

• TMB is an aromatic amine that acts as a hydrogen donor in horseradish peroxidase-catalyzed reactions
• The transfer of hydrogen results in a blue stain associated with the target of interest
• This pH-dependent color change can be confirmed using spectrophotometric methods
• TMB is commonly used in immunohistochemistry; TMB is light-sensitive, and all applications should be performed in the dark

Application

• TMB is suitable for immunohistochemistry and immunocytochemistry applications including ELISA and cell and tissue staining

Specifications

Chemical Identifiers

• CAS: 54827-17-7
• Molecular Formula: C16H20N2
• Molecular Weight (g/mol): 240.35 g/mol
• MDL Number: MFCD00007748
• InChI Key: UAIUNKRWKOVEES-UHFFFAOYSA-N
• Synonym: 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
• PubChem CID: 41206
• IUPAC Name: 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
• SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

• Color: Yellow
• Quantity: 1000 mL
• UN Number: UN2810
• Beilstein: 2808541
• Solubility Information: Insoluble in water. Soluble in organic solvents.
• Physical Form: Liquid
• Chemical Name or Material: 3,3',5,5'-Tetramethylbenzidine solution

Safety and Handling

DOTInformation	Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.
EINECSNumber	259-364-6
TSCA	Yes
Recommended Storage	Keep cold

RUO - Research Use Only