Invitrogen™ 3-Nitrotyrosine Monoclonal Antibody (2A12)

Catalog No.	Quantity
01-674-053	100 μg

Catalog No. 01-674-053 Supplier Invitrogen™ Supplier No. MA112770

Description

Mouse Monoclonal Antibody

3-Nitrotyrosine Monoclonal Antibody for Western Blot

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.

Specifications

• Antigen: 3-Nitrotyrosine
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 2A12
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 50% glycerol and 3mM sodium azide; pH 7.5
• Gene Alias: 3-Nitro-tyrosine; C9H10N2O5
• Host Species: Mouse
• Immunogen: 3-Nitrotyrosine-KLH.
• Purification Method: Protein A/G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1
• Target Molecule: Chemical