4-1BB Ligand Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P16950UG	Invitrogen™ 500P16950UG	50 μg
500P169100	Invitrogen™ 500P169100UG	100 μg
500P1691MG	Invitrogen™ 500P1691MG	1 mg

Description

AA Sequence of recombinant protein: MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human 4-1BB Ligand. Anti-Human 4-1BB Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human 4-1BB Ligand matrix. Sandwich ELISA: To detect Human 4-1BB Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human 4-1BB Ligand (500-P169Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human 4-1BB Ligand. Western Blot: To detect Human 4-1BB Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human 4-1BB Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.

Specifications

• Antigen: 4-1BB Ligand
• Applications: ELISA, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P41273
• Gene ID (Entrez): 8744
• Immunogen: E.coli-derived Recombinant Human 4-1BB Ligand.
• Purification Method: Antigen affinity chromatography
• Gene: TNFSF9

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 4 1 BB Ligand; 4 1 BBL; 41BB Ligand; 4-1BB ligand; 41BBL; 4-1BBL; 4-1BB-L; AI848817; CD 137 Ligand; CD 137L; CD137 Ligand; CD137L; Cd157l; CDw137L; costimulatory molecule; homolog of mouse 4-1BB-L; Ly63l; receptor 4-1BB ligand; TNFRSF9; Tnfsf9; TNLG5A; tumor necrosis factor (ligand) superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand 5A; tumor necrosis factor ligand superfamily member 9; tumor necrosis factor superfamily member 9
• Gene Symbols: TNFSF9
• Primary or Secondary: Primary
• Content And Storage: -20°C