Invitrogen™ 4-Hydroxy-2-hexenal Monoclonal Antibody (6F10), PerCP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545787

Catalog No. PIMA545787

Description

A 1:1,000 dilution of MA5-45787 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 μg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an alpha,beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha,beta-unsaturated hydroxyalkenal formed in this process.

Specifications

• Antigen: 4-Hydroxy-2-hexenal
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: 6F10
• Conjugate: PerCP
• Gene Alias: 4-HHE; 4-hydroxy Hexenal; 4-Hydroxy-2-hexenal; 4-Hydroxy-2-hexenal-modified; HHE; MG; MG-modified protein
• Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid
• Target Molecule: Chemical