Invitrogen™ 4-Hydroxynonenal Monoclonal Antibody (12F7), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545789

Catalog No. PIMA545789

Description

A 1:1,000 dilution of MA5-45789 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, is an alpha, beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha, beta-unsaturated hydroxyalkenal formed in this process.

Specifications

• Antigen: 4-Hydroxynonenal
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.46mM Phosphate with 2mM EDTA and no preservative; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 12F7
• Conjugate: APC
• Gene Alias: 4HNE; 4-HNE; 4-hydroxy Hexenal; 4-Hydroxy Nonenal; 4-hydroxy-2-nonenal; 4-Hydroxynonenal; HNE
• Immunogen: Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid
• Target Molecule: Chemical

Safety and Handling

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