5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody (59.1), Active Motif™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-3030	Active Motif 40000	10 μg
50-199-3031	Active Motif 40900	50 μg
50-199-3029	Active Motif 39999	100 μg

Description

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

Specifications

• Antigen: 5-Hydroxymethylcytosine (5-hmC)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 70mM tris with 0.07mM EDTA, 105mM NaCl, 30% glycerol, 31mM glycine and 0.035% sodium azide; pH 8
• Immunogen: This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to kLH and recognizes 5-hydroxymethylcytosine.
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles

• Clone: 59.1
• Applications: ChIP Assay, Dot Blot, Immunoprecipitation
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse
• Gene Alias: 5hmc; 5-hmc; 5-HydroxymethylCytidine; C5H7N3O2
• Isotype: IgG2a
• Purification Method: Protein G
• Product Type: Antibody