Invitrogen™ 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5119513

Catalog No. PIPA5119513

Description

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

Specifications

• Antigen: 5-Hydroxymethylcytosine (5-hmC)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: 5hmc; 5-hmc; 5-HydroxymethylCytidine; C5H7N3O2
• Immunogen: 5-HydroxymethylCytosine/5-hmC.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Chemical

• Applications: Dot Blot, Methylated DNA Immunoprecipitation
• Concentration: 7.65 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov