5-Methylcytosine (5-mC) Mouse anti-Human, Clone: 33D3, Active Motif™

Mouse Monoclonal Antibody

Supplier:  Active Motif 39649

Catalog No. 50-199-2759

Description

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

Specifications

• Antigen: 5-Methylcytosine (5-mC)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 10mM phosphate with 0.15M NaCl and 0.01% thimerosal; pH 7.5
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: 33D3
• Conjugate: Unconjugated
• Gene Alias: 5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
• Immunogen: Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid