Invitrogen™ 5-Methylcytosine Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585516

Catalog No. PIPA585516

Description

Keep as concentrated solution. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

Specifications

• Antigen: 5-Methylcytosine
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: 5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
• Immunogen: The immunogen used to generate this antibody corresponds to 5-MethylCytosine/ 5-mC.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Chemical

• Applications: Dot Blot, Immunomicroscopy, Methylated DNA Immunoprecipitation
• Concentration: 7.55 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG