Invitrogen™ 5-Methylcytosine Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA130675

Catalog No. PA130675

Description

The use of 7mM NaOH in the IP is crutial. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

Specifications

• Antigen: 5-Methylcytosine
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: 5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
• Immunogen: 5-methyl Cytosine coupled to coupled to keyhole limpet hemocyanin (KLH).
• Quantity: 250 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Chemical

• Applications: Dot Blot, Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 3.2 mg/mL
• Formulation: PBS with no preservative
• Host Species: Sheep
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov