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5-Methylcytosine Rabbit anti-Human, Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Manufacturer:  Invitrogen MA524694

Catalog No. PIMA524694


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Description

Description

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using Recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
Specifications

Specifications

5-Methylcytosine
Recombinant Monoclonal
1 mg/mL
PBS with 1% BSA, 50% glycerol and 0.09% sodium azide; pH 7.2-7.4
5mc; 5-mC; 5-meC; C5H7NO3; 5-Methyl-cytosine
BSA-conjugated 5-methylcytosine
Protein A
RUO
Antibody
Monoclonal
Dot blot, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
RM231
Unconjugated
Liquid
Rabbit
IgG
50 μg
-20° C, Avoid Freeze/Thaw Cycles
Primary
Human
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