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Invitrogen™ 5-Methylcytosine Recombinant Rabbit Monoclonal Antibody (RM231)
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Catalog No. PIMA524694
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50 μg
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PIMA524694 50 μg
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Catalog No. PIMA524694 Supplier Invitrogen™ Supplier No. MA524694
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Description

Rabbit Recombinant Monoclonal Antibody

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
TRUSTED_SUSTAINABILITY

Specifications

Antigen 5-Methylcytosine
Applications Dot Blot, ELISA, Flow Cytometry, Immunoprecipitation, Immunocytochemistry
Classification Recombinant Monoclonal
Clone RM231
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1% BSA, 50% glycerol and 0.09% sodium azide; pH 7.2-7.4
Gene Alias 5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3
Host Species Rabbit
Immunogen BSA-conjugated 5-methylcytosine.
Purification Method Protein A
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG
Target Molecule Chemical
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