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Invitrogen™ 6x His Tag Chimeric Recombinant Mouse Monoclonal Antibody (3D5)
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Catalog No. PIMA547746
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200 μg
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PIMA547746 200 μg
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Catalog No. PIMA547746 Supplier Invitrogen™ Supplier No. MA547746
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Description

Mouse Recombinant Monoclonal Antibody

Specificity: This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
TRUSTED_SUSTAINABILITY

Specifications

Antigen 6x His Tag Chimeric
Applications ELISA, Western Blot
Classification Recombinant Monoclonal
Clone 3D5
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.02% ProClin 300
Gene MYC
Gene Accession No. P01106
Gene Alias 6His tag; 6X His; His Tag; Histidine Tag
Host Species Mouse
Immunogen Purified his tagged Fab fragment.
Purification Method Protein A
Quantity 200 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 4609
Target Species Tag
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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