Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution

Catalog No.	Quantity
50-112-8859	2 mL

Catalog No. 50-112-8859 Supplier Invitrogen™ Supplier No. 00699350

Description

Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.

Fluorescence is detected in the far red range of the spectrum (650nm long-pass filter)

Specifications

• Color: Red
• Detection Method: Fluorescence
• For Use With (Application): Viability Assay
• For Use With (Equipment): Flow Cytometer
• Product Type: Viability Staining Solution
• Dye Type: Cell-Impermeant
• Emission: 535/617
• Product Line: eBioscience
• Quantity: 2 mL

Frequently Asked Questions (FAQs)

How should I store the eBioscience 7-AAD Viability Staining Solution (Cat. No. 00-6993-50)?

We recommend storing the eBioscience 7-AAD Viability Staining Solution (Cat. No. 00-6993-50) at 2-8 degrees C, protected from light.

How do I discriminate between live cells and dead cells when doing a flow cytometry analysis?

Discrimination between viable and non-viable cells can be carried out with the use of the 7-AAD Viability Dye (Cat. No. 00-6993-50) or Propidium Iodide (PI) Staining Solution (Cat. No. 00-6990-50). The 7-AAD or PI will mark the non-viable cells by binding to the nuclei of those cells. The nucleic acid of viable cells will not be accessible to the dye and will not be stained. When analyzing the data collected, gate out all cells stained with the viability dye. When staining for intracellular proteins, use the Fixable Viability Dye eFluor 455UV, 450, 520, 660, or 780.

For Research Use Only. Not for use in diagnostic procedures.