Invitrogen™ 7-Ketocholesterol Monoclonal Antibody (7E1), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545793

Catalog No. PIMA545793

Description

A 1:1,000 dilution of MA5-45793 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Specific for 7-ketocholesterol modified proteins.

7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies. 7-Ketocholesterol can inhibit cholesterol 7alpha-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.

Specifications

• Antigen: 7-Ketocholesterol
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.46mM phosphate with 2mM EDTA and no preservative; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 7E1
• Conjugate: APC
• Gene Alias: 7KC; 7-KC; 7KC-Modified KLH
• Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid
• Target Molecule: Chemical

Safety and Handling

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