Invitrogen™ 7-Ketocholesterol Monoclonal Antibody (7E1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527571

Catalog No. PIMA527571

Description

A 1:1,000 dilution of MA5-27571 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Specific for 7-ketocholesterol modified proteins.

7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies. 7-Ketocholesterol can inhibit cholesterol 7alpha-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.

Specifications

• Antigen: 7-Ketocholesterol
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Western Blot, Immunocytochemistry
• Clone: 7E1
• Conjugate: Unconjugated
• Gene Alias: 7KC; 7-KC; 7KC-Modified KLH
• Immunogen: Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Chemical