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LIFE TECHNOLOGIES PREMO AUTOPHAGY LC3B-GFP

Catalog No. P36235
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Catalog No. P36235 Supplier LIFE TECHNOLOGIES Supplier No. P36235

Frequently Asked Questions (FAQs)

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).
Can I use the Premo Autophagy Sensor products to study autophagy in cells in combination with a lentivirus transfection?

Lentivirus transfection may actually induce autophagy, skewing the results. Make certain to include appropriate controls.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov
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