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Invitrogen™ A-Raf Monoclonal Antibody (M289)
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Catalog No. PIMA547730
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100 μL
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PIMA547730 100 μL
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Catalog No. PIMA547730 Supplier Invitrogen™ Supplier No. MA547730
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Description

Mouse Monoclonal Antibody

This antibody detects a 68 kDa* protein corresponding to the molecular mass of A-Raf on SDS-PAGE immunoblots of human SKN-SH and Jurkat cells.

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
TRUSTED_SUSTAINABILITY

Specifications

Antigen A-Raf
Applications Western Blot
Classification Monoclonal
Clone M289
Concentration 0.125 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
Gene ARAF
Gene Accession No. P04627, P10398, P14056
Gene Alias 1200013E08Rik; Araf; A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; A-Raf proto-oncogene, serine/threonine kinase; Araf1; A-raf-1; AW495444; Oncogene ARAF1; PKS; PKS2; Proto-oncogene A-Raf; proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; Raf related protein; RAFA1; raf-related oncogene; Ras-binding protein; Ras-binding protein DA-Raf; RP1-230G1.1; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)
Gene Symbols ARAF
Host Species Mouse
Immunogen Clone (M289) was generated from a sequence corresponding to amino acids in the N-terminal region of human A-Raf. This A-Raf sequence has high homology to rat and mouse A-Raf.
Purification Method Protein G
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 11836, 369, 64363
Target Species Human, Mouse, Rat
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG1
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