Invitrogen™ AANAT Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1762

Catalog No. PA1762

Description

PA1-762 has successfully been used to detect serotonin N-AT in both WB and IF applications. PA1-762 detects serotonin N-acetyltransferase (AA-NAT) from rat tissues. PA1-762 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing unphosphorylated and phosphorylated AA-NAT from rat pineal gland whole protein extract. PA1-762 immunizing phosphopeptide (Cat. # PEP-066) corresponds to carboxy-terminal amino acid residues 198-208 of rat AA-NAT. Total anti-carboxy-terminal AA-NAT-specific IgG (Cat. # PA1-762) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-065). Phospho-specific carboxy-terminal IgG (Cat. # PA1-763) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-066). This sequence is completely conserved between rat and mouse AA-NAT protein.

Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function. Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.

Specifications

• Antigen: AANAT
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Aanat
• Gene Alias: Aanat; AA-NAT; Aralkylamine N-acetyltransferase; arylakylamine N-acetyltransferase; Arylalkylamine N - acetyltransferase (Serotonin N - acetyltransferase); arylalkylamine N-acetyltransferase; DSPS; Nat-2; Nat4; Seretonin N-acetyltransferase; serotonin acetylase; Serotonin N-acetyltransferase; SNAT
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25120
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q64666
• Gene Symbols: Aanat
• Immunogen: Synthetic Peptide: C(195) H T F L R R N S G C(205).
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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