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Description
Includes
- Human Complement C3 Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human complement C3
- Sealing Tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay
- Human Complement C3 Standard: Human Complement C3 in a buffered protein base (60μg, lyophilized)
- Biotinylated Complement C3: 1 vial, lyophilized
- EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base (30mL)
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant (30mL)
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate (μL)
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine (8mL)
- 9. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12mL)
Specifications
Specifications
| Calibration Range | 0.47 to 30μL/mL |
| Conjugate | Biotin |
| Cross Reactivity | Human |
| Description | C3 (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of human C3. |
| Detection Limit | 0.4μg/mL |
| Detection Method | Colorimetric |
| Includes | 96-well Antibody coated microwell, 60μg Human complement C3 standard, 30mL (10X) Biotinylated complement C3 1 vial, 30mL EIA diluent concentrate, 30mL Wash Buffer 20X, Streptavidin peroxidase Conjugate (SP Conjugate) 80, 8mL Chromogen Su |
| Quantity | 1 Kit |
| Sample Type | Plasma, Serum |
| Sample Volume | 25 μL |
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