Invitrogen™ ABRA1 Monoclonal Antibody (ABRA1-01)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA110045

Catalog No. PIMA110045

Description

This antibody recognizes human ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.

CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.

Specifications

• Antigen: ABRA1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: Q6UWZ7
• Gene Symbols: Abraxas1
• Immunogen: Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Clone: ABRA1-01
• Conjugate: Unconjugated
• Gene: Abraxas1
• Gene Alias: 3830405G04Rik; 5630400M01Rik; ABRA1; abraxas protein; Abraxas1; AI506069; AL024423; AV118690; BRCA1-A complex subunit Abraxas; BRCA1-A complex subunit Abraxas 1; Ccdc98; coiled-coil domain containing 98; coiled-coil domain-containing protein 98; FAM175A; family with sequence similarity 175 member A; family with sequence similarity 175, member A; FLJ11520; FLJ12642; FLJ13614; Protein FAM175A; RGD1305287; UNQ496/PRO1013
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 84142
• Content And Storage: 4°C, do not freeze
• Form: Liquid

Safety and Handling

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