Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, ROX

Catalog No. AB-1162B

Supplier: Thermo Scientific™ AB1162B

Description

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

• High Sensitivity & Specificity - The master mixes contain the patented hot start enzyme Thermo-Start DNA Polymerase
• Optimized buffer containing SYBR Green
• Reproducibility - All master mixes undergo extensive QC to ensure batch to batch consistency
• Broad dynamic range

Specifications

• Concentration: 2X
• Content And Storage: Contains:
  • 16 x 1.25 mL ABsolute QPCR SYBR Green ROX Mix (2X)
  • 100 μL MgCl₂ (1M)
  Sufficient for 1600 x 25 μL reactions.
  
  Store at -20°C
• Detection Method: SYBR
• GC-Rich PCR Performance: High
• PCR Method: qPCR
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
• Product Line: ABsolute
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 1,600 reactions
• Sufficient For: 1600 Reactions of 25 μL Each
• For Use With (Application): Gene Expression, Genotyping, siRNA Validation
• Compatibility: qPCR Cyclers
• No. of Reactions: 1,600 Reactions
• Sample Type: DNA, dsDNA
• Volume: 16 x 1.25 mL

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.